000			04644nam a22003855i 4500
001			234996
003			ES-VaUE
005			20221220020637.0
007			cr nn 008mamaa
008			151216s2016 xxu| s |||| 0|eng d
020			_a9781493933532
024	7		_a10.1007/978-1-4939-3353-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aKidney Research _bExperimental Protocols _cedited by Tim D. Hewitson, Edward R. Smith, Stephen G. Holt.
250			_a2nd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 292 páginas) _b66 ilustraciones, 14 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1397
505	0		_aIsolation and Primary Culture of Murine Podocytes with Proven Origin -- Propagation and Culture of Human Renal Fibroblasts.- Isolation and Propagation of Rat Peritoneal Mesothelial Cells.- Rat Models of Acute and/or Chronic Peritoneal Injuries Including Peritoneal Fibrosis and Peritoneal Dialysis Complications.- Renal Sympathetic Denervation in Rats.- Decellularization of Rat Kidneys to Produce Extracellular Matrix Scaffolds.- Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI.- Biopsychronology: A Method Using Live Tissue Staining to Image Cell Function in the Kidney.- Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats.- Magnetic Resonance Imaging (MRI) Analysis of Ischemia/Reperfusion in Experimental Acute Renal Injury.- Assessment of Renal Hemodynamics and Oxygenation by Simultaneous Magnetic Resonance Imaging (MRI) and Quantitative Invasive Physiological  Measurements.- Intavital Multiphoton Imaging of the Kidney: Tubular Structure and Metabolism.- Vascular Calcification in Uraemia: New-Age Concepts about an Old-Age Problem.- An In Vitro Murine Model of Vascular Smooth Muscle Cell Mineralization.- The Isolation and Quantitation of Fetuin-A Containing Calciprotein Particles from Biological Fluids.- Combining Near Infrared Fluorescent Imaging for Calcification and Inflammation in Vascular Tissue Samples Ex Vivo.- Laser Capture Microdissection of Archival Kidney Tissue for qRT-PCR -- Using Phos-Tag in Western Blotting Analysis to Evaluate Protein Phosphorylation.- Qualitative and Quantitative Analysis of Histone Deacetylases in Kidney Tissue Sections.
520			_aThis second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute kidney injury, the sympathetic nervous system, and vascular calcification. The book is divided into five parts: Part I provides a number of in vitro, in vivo, and ex vivo models of kidney disease and associated complications; Part II looks at recent advances in imaging techniques; Part III covers recent developments in studying metabolism in renal ischemia and reperfusion; Part IV addresses study and measurement of vascular calcification; and Part V explores analytical techniques that are both topical and of widespread relevance to the study of experimental renal disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Kidney Research: Experimental Protocols, Second Edition is a valuable collection of protocols useful to new and experienced researchers who are interested in the field of Nephrology.
700	1		_aHewitson, Tim D _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSmith, Edward R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHolt, Stephen G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493933518
776	0	8	_iPrinted edition: _z9781493933525
776	0	8	_iPrinted edition: _z9781493980284
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3353-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234996 _d234996