000			04567nam a22003615i 4500
001			235001
003			ES-VaUE
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007			cr nn 008mamaa
008			110225s2011 xxu| s |||| 0|eng d
020			_a9781617379987
024	7		_a10.1007/978-1-61737-998-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Immunity _bMethods and Protocols _cedited by John M. McDowell.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XV, 280 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v712
505	0		_aStudying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression -- Fragment Complementation and Co-Immunoprecipitation Assays for Understanding R Protein Structure and Function -- Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag -- Biochemical Purification of Native Immune Protein Complexes -- Chromatin Immunoprecipitation (ChIP) to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity -- Dose Response to and Systemic Movement of Dexamethasone in the GVG Inducible Transgene System in Arabidopsis -- Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis -- Constructing Haustorium-Specific cDNA Libraries from Rust Fungi -- Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes -- Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains -- A Straightforward Protocol for Electro-Transformation of Phytophthora capsici Zoospores -- Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis, a Model Oomycete Pathogen of Arabidopsis -- Assaying Effector Function In planta Using Double-Barreled Particle Bombardment -- Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast -- Purification of Effector-Target Protein Complexes via Transient Expression in N. benthamiana -- Imaging Fluorescently-Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue -- Immunolocalization of Pathogen Effectors -- Laser Capture Microdissection of Nematode Feeding Cells -- Laser Microdissection of Plant-Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling -- Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal-Plant Interaction Sites -- Visualizing Cellular Dynamics in Plant-Microbe Interactions Using Fluorescent-Tagged Proteins.
520			_aA great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes.  In  Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens.  These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity.  Written in the highly successful  Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
700	1		_aMcDowell, John M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617379970
776	0	8	_iPrinted edition: _z9781617379994
776	0	8	_iPrinted edition: _z9781493957552
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61737-998-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c235001 _d235001