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020 _a9781603279932
024 7 _a10.1007/978-1-60327-993-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMitosis
_bMethods and Protocols
_cedited by Andrew D. McAinsh.
250 _a1st edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (XI, 345 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v545
505 0 _aKnock-in and Knock-out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways -- Functional Dissection of Mitotic Regulators Through Gene Targeting in Human Somatic Cells -- RNAi in Drosophila S2 Cells as a Tool for Studying Cell Cycle Progression -- Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast -- Hydrodynamic Analysis of Human Kinetochore Complexes During Mitosis -- Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells -- Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines -- Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos -- Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells -- Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells -- Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dynamics Using GFP-Tubulin Under the Native Promoter -- Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression -- Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts -- Live Cell Imaging of Kinetochore Capture by Microtubules in Budding Yeast -- The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery -- Measuring Proteolysis in Mitosis -- An In Vitro Assay for Cdc20-Dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast -- In Vitro Assays for the Anaphase-Promoting Complex/Cyclosome (APC/C) in Xenopus Egg Extracts -- Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation -- Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Competitive (Pseudosubstrate) Inhibitors.
520 _aWith the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.
700 1 _aMcAinsh, Andrew D
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607614180
776 0 8 _iPrinted edition:
_z9781603279925
776 0 8 _iPrinted edition:
_z9781627038508
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-993-2
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2009
999 _c235002
_d235002