000 04077nam a22003615i 4500
001 235008
003 ES-VaUE
005 20221220020638.0
007 cr nn 008mamaa
008 100301s1998 xxu| s |||| 0|eng d
020 _a9781592595778
024 7 _a10.1385/0896035204
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aBioluminescence Methods and Protocols
_cedited by Robert A. LaRossa.
250 _a1st edition 1998
264 1 _aTotowa, NJ
_bHumana Press
_c1998
300 _a1 recurso en línea (X, 310 páginas)
_b33 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v102
505 0 _aThe Basics -- Improvements in the Application of Firefly Luciferase Assays -- Visualization of Bioluminescence -- Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gone -- Analytical Biochemistry -- Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue -- Bioluminescent Assay of the Guanylates -- Bioluminescent Assay of the Adenylate Energy Charge -- Molecular Biology Tools -- Photorhabdus luminescens luxCDABE Promoter Probe Vectors -- Insertion of Promoter Region::luxCDABE Fusions into the Escherichia coil Chromosome -- Probing for Promoters with Luciferase-Transposons -- Cryopreservation and Reawakening -- Luciferase Renaturation Assays of Chaperones and Chaperone Antagonists -- Cell-Based Assays -- Genotoxic Sensors -- Stress Detection Using Bioluminescent Reporters of the Heat-Shock Response -- Real-Time Reporter of Protein Synthesis Inhibition -- Luminescence-Based Cell Viability Testing -- Phagocyte Chemiluminescence -- Detection of Oxidants Using lux Fusions to Oxidative Stress Promoters -- Environmental Applications -- Luciferase-Based Measurement of Water Contaminants -- Bioluminescence-Based Metal Detectors -- Luminescence Facilitated Detection of Bioavailable Mercury in Natural Waters -- A Panel of Stress-Responsive Luminous Bacteria for Monitoring Wastewater Toxicity -- Organic Contaminant Detection and Biodegradation Characteristics -- Detection of Firefly Luciferase-Tagged Bacteria in Environmental Samples -- Monitoring of GFP-Tagged Bacterial Cells.
520 _aComprehensive and state-of-the-art, Bioluminescence Methods and Protocols brings together a collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protcols-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods for the construction of other recombinant organisms are also included. Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical, microbiological, environmental, toxicological, and analytical problems.
700 1 _aLaRossa, Robert A
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489942654
776 0 8 _iPrinted edition:
_z9780896035201
776 0 8 _iPrinted edition:
_z9781489942647
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896035204
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_1998
999 _c235008
_d235008