000			03726nam a22003855i 4500
001			235010
003			ES-VaUE
005			20221220020638.0
007			cr nn 008mamaa
008			100301s2009 xxu| s |||| 0|eng d
020			_a9781603275453
024	7		_a10.1007/978-1-60327-545-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCell-Based Assays for High-Throughput Screening _bMethods and Protocols _cedited by Paul A. Clemons, Nicola J. Tolliday, Bridget K. Wagner.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (X, 218 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v486
505	0		_aIntroduction: Cell-Based Assays for High-Throughput Screening -- High-Throughput Screening of Model Bacteria -- Cell-Based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells -- Large-Scale Small-Molecule Screen Using Zebrafish Embryos -- Whole-Animal High-Throughput Screens: The C. elegans Model -- Whole-Organism Screening: Plants -- Fluorescence-Based Assays -- Reporter Gene Assays -- Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-Restoration Assay -- Assay for Isolation of Inhibitors of Her2-Kinase Expression -- High-Content Screening: Flow Cytometry Analysis -- High-Throughput Real-Time PCR for Detection of Gene-Expression Levels -- Interpretation of Uniform-Well Readouts -- Extracting Rich Information from Images.
520			_aAs the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
700	1		_aClemons, Paul A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTolliday, Nicola J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWagner, Bridget K _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607612902
776	0	8	_iPrinted edition: _z9781603275446
776	0	8	_iPrinted edition: _z9781627039079
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-545-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c235010 _d235010