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001			235012
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007			cr nn 008mamaa
008			100301s2009 xxu| s |||| 0|eng d
020			_a9781597455572
024	7		_a10.1007/978-1-59745-557-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNucleic Acid and Peptide Aptamers _bMethods and Protocols _cedited by Günter Mayer.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XI, 408 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v535
505	0		_aI Nucleic Acid Aptamers -- Nucleic Acid Pool Preparation and Characterization -- In Vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins -- Isolating Aptamers Using Capillary Electrophoresis-SELEX (CE-SELEX) -- In Vitro Selection of Allosteric Ribozymes -- Cell-Specific Aptamers for Targeted Therapies -- Aptamers Targeting RNA Molecules -- Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interaction -- Structural Probing Techniques on Natural Aptamers -- Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography -- Locked Nucleic Acid Aptamers -- Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay -- Aptamers as Artificial Gene Regulation Elements -- Aptamers and Biosensors -- Nanoparticles/Dip Stick -- In Vivo Imaging of Oligonucleotidic Aptamers -- II Peptide Aptamers -- Selection of Peptides Interfering with Protein-Protein Interaction -- In Vitro Selection of Protein and Peptide Libraries Using mRNA Display -- Ligand-Regulated Peptide Aptamers -- Isolation of Peptide Aptamers to Target Protein Function -- Microbodies™ -- Peptide Aptamers for Small Molecule Drug Discovery -- Synthesis and Application of Peptides as Drug Carriers.
520			_aAfter the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In Nucleic Acid and Peptide Aptamers: Methods and Protocols, expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology™ series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Nucleic Acid and Peptide Aptamers: Methods and Protocols will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.
700	1		_aMayer, Günter _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781603278775
776	0	8	_iPrinted edition: _z9781934115893
776	0	8	_iPrinted edition: _z9781627038935
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-557-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c235012 _d235012