000			04059nam a22003495i 4500
001			235015
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007			cr nn 008mamaa
008			100301s2005 xxu| s |||| 0|eng d
020			_a9781592599356
024	7		_a10.1385/1592599354 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRNA Silencing _bMethods and Protocols _cedited by Gordon Carmichael.
250			_a1st edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (X, 338 páginas) _b74 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v309
505	0		_aIdentification of Components of RNAi Pathways Using the Tandem Affinity Purification Method -- Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis -- Preparation and Analysis of Drosha -- RNA Interference in Caenorhabditis elegans -- Down-Regulating Gene Expression by RNA Interference in Trypanosoma brucei -- Analysis of Double-Stranded RNA and Small RNAs Involved in RNA-Mediated Transcriptional Gene Silencing -- Strategies for the Design of Random siRNA Libraries and the Selection of anti-GFP siRNAs -- Silencing Gene Expression with Dicer-Generated siRNA Pools -- Inhibition of Gene Expression In Vivo Using Multiplex siRNA -- Gene Silencing by a DNA Vector-Based RNAi Technology -- Plasmid-Based RNA Interference -- siRNA Delivery In Vivo -- Peptide-Based Strategy for siRNA Delivery into Mammalian Cells -- Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells -- Exon-Specific RNA Interference -- Immunoprecipitation of MicroRNPs and Directional Cloning of MicroRNAs -- Detection of MicroRNAs and Assays to Monitor MicroRNA Activities In Vivo and In Vitro -- High-Throughput Analysis of MicroRNA Gene Expression Using Sensitive Probes -- Modification of Human U1 snRNA for Inhibition of Gene Expression at the Level of Pre-mRNA.
520			_aThe past decade has witnessed a true revolution in our understanding of how RNA molecules can reduce or silence gene expression, a process that holds great promise in basic research and for antiviral and anticancer applications. In RNA Silencing: Methods and Protocols, many of the most notable and distinguished researchers in the field describe in step-by-step detail their best methods for the design, preparation, and use of RNAs to reduce gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, RNA Silencing: Methods and Protocols offers today's laboratory investigators a host of optimized and validated techniques to study RNA silencing and translate its concepts into successful practical applications.
700	1		_aCarmichael, Gordon _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617375682
776	0	8	_iPrinted edition: _z9781588294364
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599354 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2005
999			_c235015 _d235015