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008			170728s2017 xxu| s |||| 0|eng d
020			_a9781493971510
024	7		_a10.1007/978-1-4939-7151-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMembrane Protein Structure and Function Characterization _bMethods and Protocols _cedited by Jean-Jacques Lacapere.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 419 páginas) _b42 ilustraciones, 13 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1635
505	0		_aRecombinant Overexpression of Mammalian TSPO Isoforms 1 and 2 -- Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex -- Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris protoplasts -- Cell-free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-synthase subunits -- Wheat Germ Cell Free Overexpression for the Production of Membrane Proteins -- Methyl-specific Isotope Labelling Strategies for NMR Studies of Membrane Proteins -- Labeling of Membrane Complexes for Electron Microscopy -- Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins Case Study of The Mouse 5-HT Receptor -- Characterization of New Detergent and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization -- Secondary Structure Determination by Means of ATR-FTIR Spectroscopy -- Native Mass Spectroscopy for the Characterization of Structure and Interactions of Membrane Proteins -- Mass Spectroscopy of Mitochondrial Membrane Proteins Complexes -- Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+NQR from V cholerae in the Presence of Lipids -- Reconstitution of Membrane Protein in Liposomes -- Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes -- The CRACAM Robot: Two Dimensional Crystallization of Membrane Protein -- Reconstitution of Membrane Proteins into Nanodiscs for Single Particle Electron Microscopy -- Solid-state NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO -- Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR -- Simulation of Ligand Binding To Membrane Proteins -- Molecular modelling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO Molecular Dynamic of Membrane Protein in Different Environments. .
520			_aIn this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field. .
700	1		_aLacapere, Jean-Jacques _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493971497
776	0	8	_iPrinted edition: _z9781493971503
776	0	8	_iPrinted edition: _z9781493983995
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7151-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235019 _d235019