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020 _a9781597455664
024 7 _a10.1007/978-1-59745-566-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aSUMO Protocols
_cedited by Helle Ulrich.
250 _a1st edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (X, 324 páginas)
_b70 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v497
505 0 _aThe SUMO System: An Overview -- Identification of Sumo Targets -- Identification of SUMO Target Proteins by Quantitative Proteomics -- Identification of SUMO-Conjugated Proteins and their SUMO Attachment Sites Using Proteomic Mass Spectrometry -- Identification of SUMO Targets Through In Vitro Expression Cloning -- Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS) -- In Vivo Detection and Characterization of Sumoylation Targets in Saccharomyces cerevisiae -- Identification Of Sumo Interactors -- Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis -- Identification of SUMO-Binding Motifs by NMR -- Functional Analysis Of Sumoylation In Vivo -- Regulation of Transcription Factor Activity by SUMO Modification -- Characterization of the Effects and Functions of Sumoylation Through Rapamycin-Mediated Heterodimerization -- Biochemical Studies Of Sumo Conjugation -- Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation -- Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes -- Preparation of Sumoylated Substrates for Biochemical Analysis -- Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli -- Biochemical Studies of Sumo Deconjugation -- Preparation of SUMO Proteases and Kinetic Analysis Using Endogenous Substrates -- An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage -- FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities -- Detection and Characterization of SUMO Protease Activity Using a Sensitive Enzyme-Based Reporter Assay -- Manipulation And Alternative Uses Of The Sumo System -- Inhibition of the SUMO Pathway by Gam1 -- SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems.
520 _aWhile the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. In SUMO Protocols, leading experts present a collection of methods intended to highlight important features unique to the biology of SUMO in order to support future SUMO research. The first half of the volume focuses on the identification and functional characterization of SUMO targets and interactors, while the second half covers essential biochemical methods for the manipulation of the enzymatic components of the SUMO system. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and the Notes sections, which provide tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, SUMO Protocols provides the necessary tools for biochemists, molecular and cellular biologists as well as research-oriented clinicians not yet familiar with this vital system.
700 1 _aUlrich, Helle
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781603279857
776 0 8 _iPrinted edition:
_z9781617379451
776 0 8 _iPrinted edition:
_z9781934115800
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-566-4
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2009
999 _c235020
_d235020