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020 _a9781627036641
024 7 _a10.1007/978-1-62703-664-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMethicillin-Resistant Staphylococcus Aureus (MRSA) Protocols
_cedited by Yinduo Ji.
250 _a2nd edition 2014
264 1 _aTotowa, NJ
_bHumana Press
_c2014
300 _a1 recurso en línea (X, 353 páginas)
_b54 ilustraciones, 24 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1085
505 0 _aClinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections -- Community-Associated Methicillin Resistant Staphylococcus aureus Case Studies -- Rapid Methods for Detection of MRSA in Clinical Specimens -- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus (MRSA) -- Internal Transcribed Spacer (ITS)-PCR Identification of MRSA -- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Isolates -- Multilocus Sequence Typing (MLST) of Staphylococcus aureus -- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA -- Genetic Interruption of Target Genes for Investigation of Virulence Factors -- Molecular Analysis of Staphylococcal Superantigens -- Investigation of Staphylococcus aureus Adhesion and Invasion of Host Cells -- Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus -- Transcriptomic Analysis of Staphylococcus aureus Using Microarray and Advanced Next-Generation RNA-seq Technologies -- Proteomic Approach to Investigate Pathogenicity and Metabolism of Methicillin-Resistant Staphylococcus aureus -- Metobolomic Investigation of Methicillin Resistant Staphylococcus aureus -- Treatment of Infections Due to Resistant Staphylococcus aureus -- Anti-Infective Drug Development for MRSA -- Animal Models in Drug Development for MRSA.
520 _aPresenting the most up-to-date techniques for the detection, genotyping, and investigation of methicillin-resistant S. aureus (MRSA), this second edition of Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols collects chapters that utilize the power of complete genomic sequences and advanced high-throughput technologies that have pushed this field to its present state.  These enable the development of specific and rapid diagnosis methods, the investigation and elucidation of mechanisms of bacterial evolution to antibiotic resistance and pathogenicity, and the identification of novel targets to develop more potent therapeutic and/or preventive agents.  Written for the Methods in Molecular Biology series, numerous chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and fully updated, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols, Second Edition serves as a key reference for researchers attempting to set up a new method to study MRSA or even for technicians and scientists working on other pathogens.
700 1 _aJi, Yinduo
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781627036658
776 0 8 _iPrinted edition:
_z9781627036634
776 0 8 _iPrinted edition:
_z9781493960569
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-664-1
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2014
999 _c235032
_d235032