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020 _a9781607618638
024 7 _a10.1007/978-1-60761-863-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMicroRNA and Cancer
_bMethods and Protocols
_cedited by Wei Wu.
250 _a1st edition 2011
264 1 _aTotowa, NJ
_bHumana Press
_c2011
300 _a1 recurso en línea (X, 273 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v676
505 0 _aMicroRNA Biogenesis and Cancer -- Computational Identification of MiRNAs Involved in Cancer -- The Principles of MiRNA-Masking Antisense Oligonucleotides Technology -- The Concept of Multiple-Target Anti-miRNA Antisense Oligonucleotide Technology -- Modulation of MicroRNAs for Potential Cancer Therapeutics -- Detection of MicroRNAs in Cultured Cells and Paraffin-Embedded Tissue Specimens by In situ Hybridization -- MicroRNA Northern Blotting, Precursor Cloning, and Ago2-Improved RNA Interference -- MiRNA Profiling on High Throughput OpenArray™ System -- Silicon Nanowire Biosensor for Ultrasensitive and Label-Free Direct Detection of miRNAs -- High Throughput and Reliable Protocols for Animal microRNA Library Cloning -- MicroRNA Regulation of Growth Factor Receptor Signalling in Human Cancer Cells -- Epigenetic Regulation of MicroRNA Expression in Cancer -- In vitro Functional Study of miR-126 in Leukemia -- Prediction of the Biological Effect of Polymorphisms within MicroRNA Binding Sites -- The Guideline of the Design and Validation of MiRNA Mimics -- Analysis of Targets and Functions Co-Regulated by MicroRNAs -- Utilization of SSCprofiler to Predict a New MiRNA Gene -- MicroRNA-Profiling Using Fluorescence-Labeled Beads: Data Acquisition and Processing.
520 _aThe tiny microRNAs (miRNAs) can have huge impacts on the regulation of a variety of genes and play crucial roles in the fundamental cellular processes. Recent miRNA studies change the landscape of cancer genetics by scrutinizing the alterations of genome-wide miRNA expressions in most common cancers and their regulatory functions during the development of cancer. The connections between miRNAs and cancer are widespread enough to warrant more comprehensive investigations in the systems biology perspective. In MicroRNA and Cancer: Methods and Protocols, internationally renowned experts provide the latest miRNA knowledge, the various techniques and methodologies currently available for cancer research application. Ranging from the fundamental concepts to practical applications, this book presents: • Overview of microRNA biogenesis, computational prediction of new miRNAs in the cancer genome, and miRNA-based therapeutic approaches for cancer treatment • Detailed experimental protocols in miRNA detection with novel and high-throughput technology, miRNA library cloning, miRNA epigenetic regulation, and miRNA pathway study • Stepwise computational and bioinformatic procedures for miRNA complex networks in cancer genomes with a variety of softwares and programs • Cross-cited notes on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols serves researchers with the basic principles of experimental and computational methods for microRNA study in cancer research and provides a firm grounding for those who wish to further develop their own applications and tailor them to their own specific research needs.
700 1 _aWu, Wei
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607618621
776 0 8 _iPrinted edition:
_z9781607618645
776 0 8 _iPrinted edition:
_z9781493957743
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-863-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2011
999 _c235041
_d235041