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020 _a9781603272605
024 7 _a10.1007/978-1-60327-260-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aRecombinant Proteins from Plants
_cedited by Charles Cunningham, Andrew J. R. Porter.
250 _a1st edition 1998
264 1 _aTotowa, NJ
_bHumana Press
_c1998
300 _a1 recurso en línea (XI, 308 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Biotechnology
_x1940-607X
_v3
505 0 _aRice Transformation by Agrobacterium Infection -- Barley as a Producer of Heterologous Protein -- Introducing and Expressing Genes in Legumes -- Use of the GUS Reporter Gene -- Expression of Recombinant Proteinase Inhibitors in Plants -- Detection of Recombinant Viral Coat Protein in Transgenic Plants -- Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants -- Tobamovirus Vectors for Expression of Recombinant Genes in Plants -- Single-Chain Fv Antibodies Expressed in Plants -- Characterization and Applications of Plant-Derived Recombinant Antibodies -- Production of Recombinant Antibodies in Plant Suspension Cultures -- Production of Foreign Proteins in Tobacco Cell Suspension Culture -- Transient Gene Expression in Plant Protoplasts -- Stability of Recombinant Proteins in Plants -- Trafficking and Stability of Heterologous Proteins in Transgenic Plants -- Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Protein -- Manipulation of Photosynthetic Metabolism -- Quantification of Heterologous Protein Levels in Transgenic Plants by ELISA -- Analysis of N- and O-Glycosylation of Plant Proteins -- Two-Dimensional Electrophoresis.
520 _aIn Recombinant Proteins from Plants, leading researchers from around the world present the latest molecular and classical approaches to the separation, analysis, and characterization of recombinant plant proteins. Focusing on the large-scale, cost-effective production of such proteins for use as specialist industrial or therapeutic biomolecules, these authoritative scientists also include promising experimental techniques that will become increasingly important in the future-such techniques as those for the efficient transformation of monocots with Agrobacterium tumefaciens, for optimizing the stability of recombinant proteins, and for exploiting the immunotherapeutic potential of plant-expressed proteins. Recombinant Proteins from Plants provides a comprehensive and detailed handbook of essential techniques that will benefit both scientists new to the field and established laboratories. It will become the standard reference for even highly skilled researchers working in this most exciting and fast developing field of biology.
700 1 _aCunningham, Charles
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aPorter, Andrew J. R
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617370212
776 0 8 _iPrinted edition:
_z9780896033900
776 0 8 _iPrinted edition:
_z9781489943033
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-260-5
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_1998
999 _c235052
_d235052