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008			150806s2016 xxu| s |||| 0|eng d
020			_a9781493926305
024	7		_a10.1007/978-1-4939-2630-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMultiple Sclerosis _bMethods and Protocols _cedited by Robert Weissert.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 232 páginas) _b37 ilustraciones, 18 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1304
505	0		_aPositional Gene Cloning in Experimental Populations -- Generation of Transgenic Rats Using Lentiviral Vectors -- DNA Vaccination Techniques -- Peripheral Blood Mononuclear Cells: Isolation, Freezing, Thawing, and Culture -- ELISPOT Techniques -- Isolation of Central Nervous System (CNS) Infiltrating Cells -- Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System -- Neurofilament Light Chain Determination from Peripheral Blood Samples -- Detection of Autoantibodies Against Myelin Oligodendrocyte Glycoprotein in Multiple Sclerosis and Related Diseases -- Myelinating Cultures: An In Vitro Tool to Identify Demyelinating and Axopathic Autoantibodies -- In Vivo Visualization of (Auto-) Immune Processes in the Central Nervous System of Rodents -- Optical Coherence Tomography to Assess Neurodegeneration in Multiple Sclerosis -- Experimental Autoimmune Encephalomyelitis in Mice -- Actively Induced Experimental Autoimmune Encephalomyelitis in Rats -- Experimental Autoimmune Encephalomyelitis in Marmosets -- Neuropathological Techniques to Investigate CNS Pathology in Experimental Autoimmune Encephalomyelitis (EAE) -- Neuropathological Techniques to Investigate Central Nervous System Sections in Multiple Sclerosis.
520			_aThis detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models. With so much effort needed to elucidate basic disease mechanisms, to clone disease relevant genes, to define novel biomarkers, and to discover novel and improved therapeutic and curative treatments, this book serves to aid researchers in accomplishing these enormous goals.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Multiple Sclerosis: Methods and Protocols will empower the reader to perform novel research regarding pathophysiology and treatment for MS.
700	1		_aWeissert, Robert _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493926299
776	0	8	_iPrinted edition: _z9781493926312
776	0	8	_iPrinted edition: _z9781493949380
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2630-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235058 _d235058