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008			161012s2017 xxu| s |||| 0|eng d
020			_a9781493940172
024	7		_a10.1007/978-1-4939-4017-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGermline Stem Cells _cedited by Michael Buszczak.
250			_a2nd edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 229 páginas) _b47 ilustraciones, 37 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1463
505	0		_aAnalysis of the C. Elegans Germline Stem Cell Pool -- Methods for Studying the Germline of the Human Parasite Schistosoma Mansoni -- Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells -- Live Imaging of the Drosophila Testis Stem Cell Niche -- RNA Isolation from Early Drosophila Larval Ovaries -- Live-Cell Imaging of the Adult Drosophila Ovary using Confocal Microscopy -- Measurement Of Mrna Poly(A) Tail Lengths In Drosophila Female Germ Cells and Germline Stem Cells -- Identification of Germline Stem Cells in Zebrafish -- Primordial Germ Cell Isolation from Xenopus Laevis Embryos -- Single-Cell Lineage Analysis of Oogenesis in Mice -- Visualization and Lineage Tracing Of Pax7+ Spermatogonial Stem Cells in the Mouse -- Transplantation as a Quantitative Assay to Study Mammalian Male Germline Stem Cells -- Mouse Fetal Germ Cell Isolation and Culture Techniques -- Rattus Norvegicus Spermatogenesis Colony Forming Assays -- Identification of Mouse Pirna Pathway Components Using Anti-MIWI2 Antibodies -- Efficient Induction and Isolation of Human Primordial Germ Cell-Like Cells from Competent Human Pluripotent Stem Cells.
520			_aThis second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter's guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.
700	1		_aBuszczak, Michael _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493940158
776	0	8	_iPrinted edition: _z9781493940165
776	0	8	_iPrinted edition: _z9781493981540
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-4017-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235061 _d235061