000			03801nam a22003615i 4500
001			235069
003			ES-VaUE
005			20221220020642.0
007			cr nn 008mamaa
008			100301s2009 xxu| s |||| 0|eng d
020			_a9781597454483
024	7		_a10.1007/978-1-59745-448-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aType 2 Diabetes _bMethods and Protocols _cedited by Claire Stocker.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (X, 278 páginas) _b56 ilustraciones, 7 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v560
505	0		_aSelecting the "Right" Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research -- Nutritional Models of Type 2 Diabetes Mellitus -- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans -- The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans -- The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ?-Cell Lines -- In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function -- Single-Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells -- Laser Capture Microdissection of Human Pancreatic ?-Cells and RNA Preparation for Gene Expression Profiling -- In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells -- The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes -- Pancreatic Remodeling: Beta-Cell Apoptosis, Proliferation and Neogenesis, and the Measurement of Beta-Cell Mass and of Individual Beta-Cell Size -- Morphology of Pancreatic Islets: A Time Course of Pre-diabetes in Zucker Fatty Rats -- Fluorescent Immunohistochemistry and In Situ Hybridization Analysis of Pancreas -- The Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent -- Hyperinsulinemic-Euglycemic Clamp to Assess Insulin Sensitivity In Vivo -- Gene Expression Analysis in Diabetes Research -- Gene Expression Mining in Type 2 Diabetes Research.
520			_aDiabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.
700	1		_aStocker, Claire _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607611486
776	0	8	_iPrinted edition: _z9781617794926
776	0	8	_iPrinted edition: _z9781934115152
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-448-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c235069 _d235069