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008			140823s2014 xxu| s |||| 0|eng d
020			_a9781493912421
024	7		_a10.1007/978-1-4939-1242-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHox Genes _bMethods and Protocols _cedited by Yacine Graba, René Rezsohazy.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XVII, 373 páginas) _b81 ilustraciones, 59 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1196
505	0		_aDiscovery and Classification of Homeobox Genes in Animal Genomes -- How to Study Hox Gene Expression and Function in Mammalian Oocytes and Early Embryos -- Genetic Lineage Tracing Analysis of Anterior Hox Expressing Cells -- A Genetic Strategy to Obtain P-Gal4 Elements in the Drosophila Hox Genes -- Hox Complex Analysis Through BAC Recombineering -- The Genetics of Murine Hox Loci: Tamere, String and Panthere to Engineer Chromosome Variants -- Topological Organization of Drosophila Hox Genes Using DNA Fluorescent In Situ Hybridization -- Mining the Cis-Regulatory Elements of Hox Clusters -- Functional Analysis of Hox Genes in Zebrafish -- Transgenesis in Non-Model Organisms: The Case of Parhyale -- Tissue Specific RNA Isolation in Drosophila Embryos: A Strategy to Analyze Context Dependent Transcriptome Landscapes Using FACS -- Hox Transcriptomics in Drosophila Embryos -- Measuring Hox-DNA Binding by Electrophoretic Mobility Shift Analysis -- Chromatin Immunoprecipitation and Chromatin Immunoprecipitation with Massively Parallel Sequencing on Mouse Embryonic Tissue -- ChIP for Hox Proteins from Drosophila Imaginal Discs -- SELEX-seq: A Method for Characterizing the Complete Repertoire of Binding Site Preferences for Transcription Factor Complexes -- DamID as an Approach to Studying Long Distance Chromatin Interactions -- cgChIP: A Cell Type and Gene Specific Method for Chromatin Analysis -- Bimolecular Fluorescence Complementation (BiFC) in Live Drosophila Embryos -- Hox Protein Interactions: Screening and Network Building -- Rational Drug Repurposing Using sscMap Analysis in a HOX-TALE Model of Leukemia.
520			_aHox Genes: Methods and Protocols explores techniques and methodologies which arose from or were successfully applied to the study of Hox genes and Hox proteins, at the intersection of experimental embryology, genetics, biochemistry, physiology, evolutionary biology, and other life sciences. This detailed volume begins with a section on discovery and functional analysis of Hox genes, and then it continues onward to discuss mode of action and biomedical applications of Hox proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Expert and practical, Hox Genes: Methods and Protocols serves as an ideal guide to researchers striving to move forward in this dynamic and exciting area of study.
700	1		_aGraba, Yacine _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRezsohazy, René _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493912438
776	0	8	_iPrinted edition: _z9781493912414
776	0	8	_iPrinted edition: _z9781493946662
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1242-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c235075 _d235075