000			04407nam a22003855i 4500
001			235076
003			ES-VaUE
005			20221220020642.0
007			cr nn 008mamaa
008			130405s2013 xxu| s |||| 0|eng d
020			_a9781627033336
024	7		_a10.1007/978-1-62703-333-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Meiosis _bMethods and Protocols _cedited by Wojciech P. Pawlowski, Mathilde Grelon, Susan Armstrong.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XV, 238 páginas) _b52 ilustraciones, 28 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029
505	0		_aSpreading and Fluorescence in situ Hybridization (FISH) of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenenetical Analysis -- Analysis of Plant Meiotic Chromosomes by Chromosome Painting -- Using Sequential Fluorescence and Genomic in situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus -- Labeling Meiotic Chromosomes in Maize with Fluorescence in situ Hybridization -- Examining Female Meiocytes of Maize by Confocal Microscopy -- Three-dimensional Acrylamide Fluorescence in situ Hybridization (FISH) for Plant Cells -- Analyzing Maize Meiotic Chromosomes with Super-resolution Structured Illumination Microscopy -- Live Imaging of Chromosome Dynamics -- Immunolocalization of Meiotic Proteins in Brassicacae - Method -- Immunolocalization of Meiotic Proteins in Arabidopsis thaliana - Method 2 -- Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana - Method 3 -- A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana -- Analyzing Meiotic Chromosomes in Rice -- Analysing Meiosis in Barley -- Preparing SC Spreads with RNs for EM Analysis -- Analysis of the Synaptonemal Complex in Brassica using TEM -- Preparing Thin Sections of Meiotic Nuclei for Transmission Electron Microscopy -- Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen -- Chromatin Immunoprecipitation for Studying Chromosomal Localization of Meiotic Proteins in Maize -- Analyzing the Meiotic Transcriptome using Isolated Meiocytes of Arabidopsis thaliana -- Analysis of Meiotic Protein Complexes from Arabidopsis and Brassica using Affinity-based Proteomics -- Identifying Meiotic Mutants in Arabidopsis thaliana.
520			_aMeiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation.  In plants, meiotic recombination is by far the most important source of genetic variation.  In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants.  These state-of -the-art protocols allow studying the organization and behavior of the genetic material in a wide range of both model and crop species.  Written in the highly successful Methods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.    Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.
700	1		_aPawlowski, Wojciech P _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aGrelon, Mathilde _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aArmstrong, Susan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627033329
776	0	8	_iPrinted edition: _z9781627033343
776	0	8	_iPrinted edition: _z9781493959723
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-333-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c235076 _d235076