000			03814nam a22003615i 4500
001			235077
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007			cr nn 008mamaa
008			100301s1999 xxu| s |||| 0|eng d
020			_a9781592594863
024	7		_a10.1385/0896035476 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
100	1		_aJenkins, Nigel. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aAnimal Cell Biotechnology _bMethods and Protocols _cby Nigel Jenkins.
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XIV, 302 páginas) _b43 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Biotechnology _x1940-607X _v8
505	0		_aEnabling Technologies -- Setting Up a New Cell Culture Laboratory -- Adaptation of Mammalian Cells to Growth in Serum-Free Media -- Viral Evaluation of Animal Cell Lines Used in Biotechnology -- Molecular Methods -- Optimizing Gene Expression in Mammalian Cells -- Cytogenetic Characterization of Recombinant Cells -- Immortalization Strategies for Mammalian Cells -- Mouse Monoclonal Antibodies -- Cell Bank Preparation and Characterization -- DNA Fingerprinting and Characterization of Animal Cells -- Cell Evaluation Protocols -- Cell Counting and Viability Measurements -- Monitoring Animal Cell Growth and Productivity by Flow Cytometry -- Measurement of Cell Death in Culture -- Nuclear Magnetic Resonance Methods of Monitoring Cell Metabolism -- Specialist Techniques -- Culturing Animal Cells in Fluidized Bed Reactors -- GPI-Anchored Fusion Proteins -- Harvesting GPI-Anchored Proteins From CHO Cells -- Hematopoietic Cells for Cellular and Gene Therapy -- Hematopoietic Cells for Cellular and Gene Therapy -- Cytotoxicity Testing Using Cell Lines -- Product Evaluation Protocols -- Measuring the Folding Dynamics of Recombinant Proteins Secreted from Mammalian Cells -- Control of Proteolysis in Cell Culture -- Monitoring Recombinant Glycoprotein Heterogeneity -- Measurement of Cell-Culture Glycosidase Activity.
520			_aIn Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases.
776	0	8	_iPrinted edition: _z9781489943583
776	0	8	_iPrinted edition: _z9780896035478
776	0	8	_iPrinted edition: _z9781489943576
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896035476 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1999
999			_c235077 _d235077