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020 _a9781592591480
024 7 _a10.1385/1592591485
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMeningococcal Vaccines
_bMethods and Protocols
_cedited by Andrew J. Pollard, Martin C.J. Maiden.
250 _a1st edition 2001
264 1 _aTotowa, NJ
_bHumana Press
_c2001
300 _a1 recurso en línea (XVII, 421 páginas)
_b20 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Medicine
_x1940-6037
_v66
505 0 _aMeningococcal Vaccines and Vaccine Developments -- Immune Response and Host-Pathogen Interactions -- Purification of Capsular Polysaccharide -- Protein-Polysaccharide Conjugation -- N-Propionylation -- Outer Membrane Protein Purification -- Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease -- Methods for Manipulation of Transferrin-Binding Proteins -- Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. meningitidis Outer Membrane Proteins -- Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics -- Construction of porA Mutants -- Construction of LPS Mutants -- Recombinant Proteins in Vaccine Development -- Identification of Peptides that Mimic N. meningitidis LOS Epitopes Via the Use of Combinatorial Phage-Display Libraries -- Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity -- Early Experiences with Nasal Vaccines against Meningococcal Disease -- Animal Models for Meningococcal Disease -- Determination of Antibody Responses to Meningococcal Antigens by ELISA -- Immunoblot Analysis of Sera from Patients and Vaccinees -- Serogroup B and C Serum Bactericidal Assays -- A Modified ELISA for Measurement of High-Avidity IgG Antibodies to Meningococcal Serogroup C Polysaccharide that Correlate with Bactericidal Titers -- Whole-Blood Model -- Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry -- T-Cell Responses Against Meningococcal Antigens -- CD4 T-Cell Epitope Mapping -- Epitope Mapping -- Meningococcal Vaccine Trials -- The Introduction of Group C Conjugate Meningococcal Vaccine into the UK.
520 _aThe development of a comprehensive vaccine against Neisseria meningitidis, the causative agent of meningococcal disease, has remained elusive because of bacterial diversity and immunologic evasion. In Meningococcal Vaccines: Methods and Protocols, Andrew Pollard and Martin Maiden have assembled an impressive collection of the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines to be used against this dreaded disease. The contributors-leading scientists, clinicians, and public health physicians-describe in detail the many approaches to vaccine design, as well as the assessment of immune response to vaccine candidates and novel vaccine formulations. They also present as a test case the recent implementation of a new meningococcal vaccine in the United Kingdom. A companion volume, Meningococcal Disease: Methods and Protocols, contains detailed methods for diagnostic microbiology, bacterial characterization, epidemiology, host-pathogen interactions, and clinical studies. Timely and comprehensive, Meningococcal Vaccines: Methods and Protocols provides the scientist, public health physician, epidemiologist, clinical microbiologist, and clinician with the essential tools to lay bare the secrets of the meningococcus and to develop, evaluate, and implement successful new meningococcal vaccines.
700 1 _aPollard, Andrew J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMaiden, Martin C.J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489942258
776 0 8 _iPrinted edition:
_z9780896038011
776 0 8 _iPrinted edition:
_z9781489942241
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591485
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2001
999 _c235085
_d235085