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001			235087
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008			100301s2003 xxu| s |||| 0|eng d
020			_a9781592593774
024	7		_a10.1385/1592593771 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDiabetes Mellitus _cedited by Sabire Özcan.
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (X, 192 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v83
505	0		_aInsulin Production -- Isolation of Islets of Langerhans from Rodent Pancreas -- Purification of Rat Pancreatic ?-Cells by Fluorescence-Activated Cell Sorting -- Assessment of Insulin Secretion in the Mouse -- Detection of Insulin Production by Immunohistochemistry -- Quantification of the Level of Insulin Gene Expression -- Chromatin Immunoprecipitation Using Isolated Islets of Langerhans -- Adenoviral Gene Transfer into ?-Cell Lines -- Utilization of NOD Mice in the Study of Type 1 Diabetes -- Insulin Action -- of DNA into 3T3-L1 Adipocytes by Electroporation -- Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes -- Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes -- Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay -- Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates -- Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity -- Assaying AKT/Protein Kinase B Activity -- Measurements of Cellular Phosphoinositide Levels in 3T3-L1 Adipocytes -- Measurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 Adipocytes -- Measurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal Muscle -- Single Embryo Measurement of Basal-and Insulin-Stimulated Glucose Uptake -- Immunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of Glucose Transporters.
520			_aAbout 17 million Americans suffer from diabetes mellitus, a disease caused by defects in insulin production, insulin action, or both, and also a leading cause of kidney failure, blindness, and amputations. In Diabetes Mellitus: Methods and Protocols, leading researchers and clinical investigators describe their cutting-edge techniques for studying these defects at both the molecular and biochemical levels. Written in step-by-step detail to ensure ready reproducibility and robust results, these techniques allow investigators, both novices and those already active in the field, to study every major facet of insulin production and action. Each protocol includes an introduction to the technique, an explanation of its application, and a list of materials. Practical notes discuss how to avoid pitfalls, as well as how to adapt the methods to your own research. Cutting-edge and highly practical, Diabetes Mellitus: Methods and Protocols provides basic scientists and clinical researchers, both experienced investigators and those new to the field, all the critical tools needed for the productive study of diabetes mellitus today.
700	1		_aÖzcan, Sabire _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617373886
776	0	8	_iPrinted edition: _z9781588291486
776	0	8	_iPrinted edition: _z9781489943644
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593771 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2003
999			_c235087 _d235087