000			04693nam a22003615i 4500
001			235095
003			ES-VaUE
005			20221220020643.0
007			cr nn 008mamaa
008			120913s2012 xxu| s |||| 0|eng d
020			_a9781627030328
024	7		_a10.1007/978-1-62703-032-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSingle-Stranded DNA Binding Proteins _bMethods and Protocols _cedited by James L. Keck.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (X, 259 páginas) _b40 ilustraciones, 20 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v922
505	0		_aFunctions of Single-Strand DNA-Binding Proteins in DNA Replication, Recombination and Repair.-Structural Diversity Based on Variability in Quaternary Association. A Case Study Involving Eubacterial and Related SSBs -- SB Binding to ssDNA Using Isothermal Titration Calorimetry -- SSB-DNA Binding Monitored by Fluorescence Intensity and Anisotropy -- Single Molecule Analysis of SSB Dynamics on Single Stranded DNA -- Sample Preparation Methods to Analyze DNA-induced Structural Changes in Replication Protein A (RPA) -- Structural Studies of SSB Interaction with RecO -- Investigation of Protein-Protein Interactions of Single-Stranded DNA-Binding Proteins by Analytical Ultracentrifugation -- Ammonium Sulfate Co-Precipitation of SSB and Interacting Proteins -- Analyzing Interactions Between SSB and Proteins by the Use of Fluorescence Anisotropy -- Far Western Blotting as a Rapid and Efficient Method for Detecting Interactions Between DNA Replication and DNA Repair Proteins -- Methods for Analysis of SSB/Protein Interactions by SPR -- Use of Native Gels to Measure Protein Binding to SSB -- Identification of Small Molecules That Disrupt SSB/Protein Interactions Using a High-Throughput Screen -- Detection of Post-translational Modifications of Replication Protein A -- Detecting Posttranslational Modification of Bacterial SSB Proteins -- Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA -- Fluorescent Single-Stranded DNA-Binding Proteins Enable in vitro and in vivo Studies< -- Use of Fluorescently Tagged SSB Proteins in in vivo Localization Experiments.
520			_aThe genomes of cellular organisms are organized as double-stranded DNA, a structure that must be unwound to provide DNA replication, recombination, and repair machinery access to genomic information.  However, DNA unwinding comes with inherent risks to genome stability.  To help mediate these risks, bacterial, archael, and eukaryotic cells have evolved protective ssDNA-binding proteins (SSBs) that bind ssDNA with high affinity and specificity.  SSBs also aid genome metabolic processes through direct interactions with key proteins in genome maintenance enzymes.  Single-Stranded DNA Binding Proteins: Methods and Protocols assembles methods developed for examining the fundamental properties of SSBs and for exploiting the biochemical functions of SSBs for their use as in vitro and in vivo reagents. Clearly and concisely organized, the volume opens with an introduction to the structures and functions of SSBs, followed protocols for studying SSB/DNA complexes, methods for studying SSB/heterologous protein complexes, protocols for interrogating post-translational modifications of SSBs, and concludes with uses of fluorescently-labeled SSBs for in vitro and in vivo studies of genome maintenance processes.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Single-Stranded DNA Binding Proteins: Methods and Protocols provides a rich introduction for investigators who are interested in this fascinating family of DNA-binding proteins.
700	1		_aKeck, James L _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627030335
776	0	8	_iPrinted edition: _z9781627030311
776	0	8	_iPrinted edition: _z9781493962518
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-032-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c235095 _d235095