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008			140517s2014 xxu| s |||| 0|eng d
020			_a9781493905546
024	7		_a10.1007/978-1-4939-0554-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEngineering and Analyzing Multicellular Systems _bMethods and Protocols _cedited by Lianhong Sun, Wenying Shou.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 256 páginas) _b109 ilustraciones, 86 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1151
505	0		_aRecent Progress in Engineering Human-associated Microbiomes -- Constructing Synthetic Microbial Communities to Explore the Ecology and Evolution of Symbiosis -- Combining Engineering and Evolution to Create Novel Metabolic Mutualisms Between Species -- Design, Construction and Characterization Methodologies for Synthetic Microbial Consortia -- An Observation Method for Autonomous Signaling-mediated Synthetic Diversification in Escherichia coli -- Integration-free Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells (iPSCs) without Viral Vectors, Recombinant DNA and Genetic Modification -- Transformation of Bacillus subtilis -- Culturing Anaerobes to use as a Model System for Studying the Evolution of Syntrophic Mutualism -- Therapeutic Microbes for Infectious Disease -- Quantitative Measurement and Analysis in a Synthetic Pattern Formation Multicellular System -- Transcriptome Analysis of a Microbial Coculture in which the Cell Populations are Separated by a Membrane -- Identification of Mutations in Laboratory Evolved Microbes from Next-generation Sequencing data using breseq -- 3D-Fluorescence in situ Hybridization of Intact, Anaerobic Biofilm -- The Characterization of Living Bacterial Colonies Using Nano spray Desorption Electrospray Ionization Mass Spectrometry -- Modeling Community Population Dynamics with the Open-Source Language R -- Simulating Microbial Community Patterning using Biocellion.
520			_aEngineering Multicellular Systems: Methods and Protocols, focuses on laboratory procedures used in recent efforts for constructing synthetic multicellular systems and their applications. In particular, constructing multicellular systems to form various microbial ecosystems has been extensively explored to examine evolution and interactions of microbial ecosystems, while co-cultures have emerged as an efficient tool to produce some complex chemical molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Engineering Multicellular Systems: Methods and Protocols provides a comprehensive laboratory protocol reference for constructing multicellular systems for various applications.
700	1		_aSun, Lianhong _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aShou, Wenying _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493905553
776	0	8	_iPrinted edition: _z9781493905539
776	0	8	_iPrinted edition: _z9781493952724
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0554-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c235098 _d235098