000			03433nam a22003735i 4500
001			235109
003			ES-VaUE
005			20221220020644.0
007			cr nn 008mamaa
008			170103s2017 xxu| s |||| 0|eng d
020			_a9781493967223
024	7		_a10.1007/978-1-4939-6722-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aXylem _bMethods and Protocols _cedited by Miguel de Lucas, J. Peter Etchhells.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 262 páginas) _b50 ilustraciones, 37 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1544
505	0		_aExperimental and Theoretical Methods to Investigate Vascular Development in Plants -- Strigolactone-Mediated Stimulation of Secondary Xylem Proliferation in Stems -- Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues -- Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis -- Tissue Culture for Xylem Differentiation with Arabidopsis Leaves -- VND6-Induced Xylem Cell Differentiation in Arabidopsis Cell Cultures -- Live Imaging of Developing Xylem in Planta -- Immunolocalization in Secondary Xylem of Populus -- Monitoring Vascular Regeneration and Xylem Connectivity In Arabidopsis Thaliana -- Vascular Morphodynamics during Secondary Growth -- Improved Mps-PI Staining for Xylem Characterization -- Chemical Imaging of Xylem by Raman Microspectroscopy -- Using Cellprofiler to Analyse and Quantify Vascular Morphology -- Lignin Analysis by HPLC and FTIR -- Carbohydrate Composition Analysis in Xylem -- Structural Analysis of Cell Wall Polysaccharides Using PACE -- Analysis of Lignin Composition and Distribution Using Fluorescence Laser Confocal Microspectroscopy. .
520			_aThis volume provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Xylem: Methods and Protocols, aims to ensure successful results in the further study of this vital field.
700	1		_ade Lucas, Miguel _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aEtchhells, J. Peter _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493967209
776	0	8	_iPrinted edition: _z9781493967216
776	0	8	_iPrinted edition: _z9781493982820
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6722-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c235109 _d235109