000			03220nam a22003615i 4500
001			235115
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007			cr nn 008mamaa
008			150219s2015 xxu| s |||| 0|eng d
020			_a9781493923656
024	7		_a10.1007/978-1-4939-2365-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPCR Primer Design _cedited by Chhandak Basu.
250			_a2nd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XII, 216 páginas) _b73 ilustraciones, 45 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1275
505	0		_aFast Masking Of Repeated Primer Binding Sites In Eukaryotic Genomes -- Primer Design for PCR Reactions in Forensic Biology -- Design of Primers and Probes For Quantitative Real-Time PCR Methods -- Large-scale Nucleotide Sequence Alignment And Sequence Variability Assessment To Identify the Evolutionarily Highly Conserved Regions For Universal Screening PCR Assay Design: An Example Of Influenza A Virus -- Low-concentration Initiator Primers Improve The Amplification Of Gene Targets With High Sequence Variability -- Multiplex PCR Primer Design for Simultaneous Detection of Multiple Pathogens -- Degenerate Primer Design for Highly Variable Genomes -- Allele-specific Real Time Polymerase Chain Reaction As A Tool For Urate Transporter 1 Mutation Detection -- MultiPLX: Automatic Grouping And Evaluation Of PCR Primers -- In silico PCR Primer Designing and Validation -- Primer Design using Primer Express® for SYBR Green Based Quantitative PCR -- Designing Primers for SNaPshot Technique -- Rapid and Simple Method of qPCR Primer Design -- PRIMEGENSw3: A Web-based Tool for High-throughput Primer and Probe Design -- Selecting Specific PCR Primers with MFEprimer.
520			_aThis volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.
700	1		_aBasu, Chhandak _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493923663
776	0	8	_iPrinted edition: _z9781493923649
776	0	8	_iPrinted edition: _z9781493947553
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2365-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c235115 _d235115