000			04178nam a22003975i 4500
001			235124
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007			cr nn 008mamaa
008			100427s2010 xxu| s |||| 0|eng d
020			_a9781607610632
024	7		_a10.1007/978-1-60761-063-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtocols for Adult Stem Cells _cedited by Irina M. Conboy, David V. Schaffer, Mary Helen Barcellos-Hoff, Song Li.
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (X, 207 páginas) _b20 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v621
505	0		_aIn Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells -- Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies -- Use of Stem Cell Markers in Dissociated Mammary Populations -- Isolation of Adult Hippocampal Neural Progenitors -- In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors -- In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors -- Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors -- Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix -- Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis -- Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells -- Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells -- Altered Expression of NFkB in Ex Vivo Differentiated Dendritic Cells from the Aged Subjects: Implications in Immunotherapy -- Preparation of Collagen-Coated Gels that Maximize In Vitro Myogenesis of Stem Cells by Matching the Lateral Elasticity of In Vivo Muscle.
520			_aThe study of adult stem cells has surged in recent years. Because they are responsible for the body's natural ability to fight diseases, heal and recover, or fail and succumb to various maladies, it has become increasingly important to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. In Protocols for Adult Stem Cells, expert researchers present a variety of methods for studying five types of clinically-relevant mammalian stems cells: mammary, nerve, skeletal muscle, endothelial and mesenchymal. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Chapters include information that will assist researchers in obtaining, characterizing and studying these cells or adapting them to the stem cells of choice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Critical and cutting edge, Protocols for Adult Stem Cells is an essential guide which provides groundbreaking and novel techniques certain to redefine the field of stem cell biology.
700	1		_aConboy, Irina M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSchaffer, David V _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBarcellos-Hoff, Mary Helen _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLi, Song _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607615088
776	0	8	_iPrinted edition: _z9781607610625
776	0	8	_iPrinted edition: _z9781493961047
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-063-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2010
999			_c235124 _d235124