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007			cr nn 008mamaa
008			130822s2013 xxu| s |||| 0|eng d
020			_a9781627036283
024	7		_a10.1007/978-1-62703-628-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEpiblast Stem Cells _bMethods and Protocols _cedited by Ramiro Alberio.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 216 páginas) _b49 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1074
505	0		_aGeneration and Characterization of Epiblast Stem Cells from Blastocyst-stage Mouse Embryos -- Reversion of Mouse Post-Implantation Epiblast Stem Cells to a Naïve Pluripotent State by Modulation of Signalling Pathways -- Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by using Small Molecules -- Isolation and Culture of Rabbit Embryonic Stem Cells -- Goat Embryonic Stem-like Cell Derivation and Characterization -- Derivation and Culture of Canine Embryonic Stem Cells -- Isolation and Culture of Porcine Embryonic Stem Cells -- Isolation and Culture of Pig Epiblast Stem Cells -- Isolation and Culture of Bovine Embryonic Stem Cells -- Analysis of Molecular Markers for Staging Peri-gastrulating Bovine Embryos -- Chicken Embryonic Stem Cells: Establishment and Characterization -- Manipulating the Avian Epiblast and Epiblast-derived Stem Cells -- Stepwise Differentiation from Naïve State Pluripotent Stem Cells to Functional Primordial Germ Cells through an Epiblast-like State -- Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells -- Derivation of Neural Precursors from Bovine Pre-implantation Embryos.
520			_aIn Epiblast Stem Cells: Methods and Protocols, expect researchers in the field provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast.  These include methods and techniques used to study epiblast development in different amniotes. This collection brings together contributions from the fields of embryology, stem cell biology and developmental biology together, providing a single volume with detailed procedures for the isolation and culture of epiblasts at different stages of development, and techniques for the study of differentiation into specific lineages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, a complete list of the necessary materials and reagents, detailed laboratory protocols, and extensive notes providing suggestions on troubleshooting and how to overcome common difficulties.   Comprehensive and cutting-edge, Epiblast Stem Cells: Methods and Protocols serves as a resource to individuals interested in studying the biology of pluripotent cells.
700	1		_aAlberio, Ramiro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627036290
776	0	8	_iPrinted edition: _z9781627036276
776	0	8	_iPrinted edition: _z9781493960330
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-628-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c235126 _d235126