000			03651nam a22003735i 4500
001			235136
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592591824
024	7		_a10.1385/1592591825 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDNA Methylation Protocols _cedited by Ken I. Mills, Bernie H. Ramsahoye.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (X, 189 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v200
505	0		_aOverview -- Nearest-Neighbor Analysis -- Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography -- Methylation Analysis by Chemical DNA Sequencing -- Methylation-Sensitive Restriction Fingerprinting -- Restriction Landmark Genome Scanning -- Combined Bisulfite Restriction Analysis (COBRA) -- Differential Methylation Hybridization Using CpG Island Arrays -- Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences -- Isolation of CpG Islands Using a Methyl-CpG Binding Column -- Purification of MeCP2-Containing Deacetylase from Xenopus laevis -- DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method -- Measuring DNA Demethylase Activity In Vitro -- Extracting DNA Demethylase Activity from Mammalian Cells.
520			_aSince methylation is now understood to be integral to DNA replication and transcription, with abnormalities frequently found to occur during tumorigenesis, the number of breakthrough techniques for methylation's study has sharply increased. In DNA Methylation Protocols, an internationally well-recognized panel of investigators offer a set of readily reproducible protocols for the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation, and the isolation and measurement of demethylases and related proteins. Each technique is described by a hands-on master, and includes a concise summary of the basic theory, a complete materials list, step-by-step instructions for its successful execution, and helpful notes on avoiding pitfalls. Comprehensive and cutting-edge, DNA Methylation Protocols brings together not only the basic methods, but also the many exciting new techniques available today for the analysis of gene- and site-specific DNA methylation, and will prove invaluable to all researchers investigating replication, transcription, growth, differentiation, and carcinogenesis today.
700	1		_aMills, Ken I _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRamsahoye, Bernie H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489938848
776	0	8	_iPrinted edition: _z9781489938831
776	0	8	_iPrinted edition: _z9780896036185
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591825 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c235136 _d235136