000			04518nam a22003735i 4500
001			235150
003			ES-VaUE
005			20221220020647.0
007			cr nn 008mamaa
008			100301s1991 xxu| s |||| 0|eng d
020			_a9781592594948
024	7		_a10.1385/0896031780 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
100	1		_aMurray, Edward J. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aGene Transfer and Expression Protocols _cby Edward J. Murray.
250			_a1st edition 1991
264		1	_aTotowa, NJ _bHumana Press _c1991
300			_a1 recurso en línea (XIII, 439 páginas) _b33 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v7
505	0		_aPreparation of Recombinant Plasmid DNA for DNA-Mediated Gene Transfer -- Calcium Phosphate Mediated Gene Transfer into Established Cell Lines -- Transfection of the ChloramphenicolAcetyltransferase Gene into Eukaryotic Cells Using Diethyl-Aminoethyl (DEAE)-Dextran -- Polybrene/DMSOAssisted Gene Transfer -- Electroporation Technique of DNA Transfection -- Irradiation and Fusion Gene Transfer -- Direct Use of ? Phage Particles for DNA Transfection -- Cationic Liposome-Mediated Transfection with Lipofectin™ Reagent -- Preparation of High-Molecular-Weight DNA for Use in DNA Transfection -- Chromosome-Mediated Gene Thnsfer -- Manipulation of Adenovirus Vectors -- Manipulation of Vaccinia Virus Vectors -- Manipulation of Baculovirus Vectors -- Manipulation of SV40 Vectors -- Choice and Manipulation of Retroviral Vectors -- Use of Tissue-Plasminogen Activator as a Reporter Gene -- Use of Escherichiu coli (E. coli) lacZ (?-Galactosidase) as a Reporter Gene -- Application of the Firefly Luciferase Reporter Gene -- Use of Vectors to Confer Resistance to Antibiotics G418 and Hygromycin in Stably Transfected Cell Lines -- Selection of Cells Defective in Pyrimidine (TK?) and Purine (APRT? and HPRT? Salvage -- Quantitative and Qualitative Analysis of Exogenous Gene Expression by the S1 Nuclease ProtectionAssay -- The RNase Protection Assay -- Primer Extension Analysis of mRNA Isolated from Transfected Cell Lines -- Northern Blot Analysis of Gene Expression -- Analysis of Transcriptional Initiation in Isolated Nuclei -- Immunoperoxidase Staining of Gene Products in Cultured Cells Using Monoclonal Antibodies -- The Use of Flow Cytometry to Detect Transfected Gene Products -- Determination of Foreign Gene Copy Number in Stably Transfected Cell Lines by Southern Transfer Analysis -- Evaluation of Extrachromosomal Gene Copy Number of Transiently Transfected Cell Lines -- Use of Dpn I Restriction Enzyme to Assess Newly Replicated Gene Copies in Amplifiable Vector Systems -- Use of Polymerase Chain Reaction (PCR) to Detect Homologous Recombination in Transfected Cell Lines -- Induction of Erythroid-Specific Expression in Murine Erythroleukemia (MEL) Cell Lines.
520			_aBiology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .
776	0	8	_iPrinted edition: _z9781489944030
776	0	8	_iPrinted edition: _z9781489944023
776	0	8	_iPrinted edition: _z9780896031784
776	0	8	_iPrinted edition: _z9780896032163
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896031780 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1991
999			_c235150 _d235150