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020 _a9781493926206
024 7 _a10.1007/978-1-4939-2620-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Pathology
_bTechniques and Protocols
_cedited by Christophe Lacomme.
250 _a2nd edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XI, 352 páginas)
_b67 ilustraciones, 47 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1302
505 0 _aDetection of the Bacterial Potato Pathogens Pectobacterium and Dickeya spp. Using Conventional and Real Time PCR -- Detection and Identification of Phoma Pathogens of Potato -- Diagnosis of Ramularia collo-cygni and Rhynchosporium spp in Barley -- A Real-Time Multiplex PCR Assay Used in the Identification of Closely Related Fungal Pathogens at the Species Level -- Diagnostics of Tree Diseases Caused by Phytophthora Species -- Real-Time LAMP for Chalara fraxinea Diagnosis -- Loop-Mediated Isothermal Amplification Procedure (LAMP) for Detection of the Potato Zebra Chip Pathogen 'Candidatus Liberibacter Solanacearum' -- Loop-Mediated Isothermal Amplification (LAMP) for Detection of Phytoplasmas in the Field -- Diagnosis of Phytoplasmas by Real-Time PCR Using Locked Nucleic Acid (LNA) Probes -- Q-Bank Phytoplasma: A DNA Bar-Coding Tool for Phytoplasma Identification -- High-Throughput Diagnosis of Potato Cyst Nematodes in Soil Samples -- Detection of Nepovirus Vector and Non-Vector Xiphinema Species in Grapevine -- Molecular and Serological Methods for the Diagnosis of Viruses in Potato Tubers -- Immunocapture-Multiplex RT-PCR for the Simultaneous Detection and Identification of Plant Viruses and Their Strains: Study Case, Potato Virus Y (PVY) -- Snapshot and CE-SSCP: Two Simple and Cost-Effective Methods to Reveal Genetic Variability within a Virus Species -- Detection and Characterization of Viral Species/Sub-Species Using Isothermal Recombinase Polymerase Amplification (RPA) Assays -- Virus-Testing by PCR and RT-PCR Amplification in Berry Fruit -- Metagenomics Approaches Based on Virion-Associated Nucleic Acids (VANA): An Innovative Tool for Assessing without a Priori the Viral Diversity of Plants -- Detection of Potato spindle tuber viroid and Other Related Viroids by a DIG Labeled RNA Probe -- Microarray Platform for the Detection of a Range of Plant Viruses and Viroids -- Multiplex Detection of Plant Pathogens Through the Luminex Magplex Bead System -- Next Generation Sequencing of Elite Berry Germplasm and Data Analysis Using a Bioinformatics Pipeline for Virus Detection and Discovery -- Metagenomic Next-Generation Sequencing of Viruses Infecting Grapevines -- Droplet Digital PCR for Absolute Quantification of Pathogens.
520 _aThe second edition of Plant Pathology: Techniques and Protocols covers diagnostic methods that are currently used in laboratories for a broad range of plant species and matrixes. These include serological and molecular methods that have one or more of the following characteristics: suitability for high-throughput testing, detection of a group of pathogens or of sometimes uncharacterized pathogens, detection and identification of specific pathogens, and high sensitivity. This volume discusses qualitative and quantitative tests, as well as recently developed diagnostic methods. It also provides background information on many pathogens, which are either endemic, non-endemic, or emerging and with different lifecycles that cause diseases of significant importance in a wide variety of hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, background information on pathogens and the disease caused, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Plant Pathology: Techniques and Protocols, Second Edition is the perfect book for plant pathologists and molecular biologists who will use this information to test out the latest research in their laboratories.
700 1 _aLacomme, Christophe
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493926213
776 0 8 _iPrinted edition:
_z9781493926190
776 0 8 _iPrinted edition:
_z9781493949250
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2620-6
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2015
999 _c235154
_d235154