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001			235164
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007			cr nn 008mamaa
008			160830s2016 xxu| s |||| 0|eng d
020			_a9781493937929
024	7		_a10.1007/978-1-4939-3792-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Nucleolus _bMethods and Protocols _cedited by Attila Németh.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 279 páginas) _b55 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1455
505	0		_aThe Relationship between Human Nucleolar Organizer Regions and Nucleoli, Probed by 3D-Immunofish -- Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces Cerevisiae -- High-Throughput Live-Cell Microscopy Analysis of Chromosome Domain - Nucleolus Associations in S. Cerevisiae -- Quantitative Immunofluorescence Analysis of Nucleolus-Associated Chromatin -- Visualization of The Nucleolus in Living Cells with Cell-Penetrating Fluorescent Peptides -- Purification of S. Cerevisiae RNA Polymerase I for Structural and Functional Studies -- Analysis of S. Cerevisiae RNA Polymerase I Transcription In Vitro -- Preparation of Chromatin Templates to Study RNA Polymerase I Transcription In Vitro -- Measurement of Rrna Synthesis and Processing by 4su-Tagging -- Metabolic Labeling in the Study of Mammalian Ribosomal RNA Synthesis -- Quantitative Northern Blot Analysis of Mammalian Rrna Processing -- Complete Sequence Construction of the Highly Repetitive Ribosomal RNA Gene Repeats in Eukaryotes using Whole Genome Sequence Data -- Analysis of Rdna Methylation in Arabidopsis By 2D Gel Electrophoresis -- Fluorescence-Activated Sorting of Arabidopsis Nucleoli -- Purification of RNA Polymerase I-Associated Chromatin from Yeast Cells -- Bioinformatic Analysis of Chip-Seq Data on the Repetitive Rrna Gene -- Deep Sequencing Analysis of Nucleolar Small Rnas: Isolation and Library Preparation -- Deep Sequencing Analysis of Nucleolar Small RNAs: Bioinformatics -- Quantitative Proteomic Analysis of the Human Nucleolus -- Assessing Nucleolar Localization of Proteins: Bioinformatic Analysis of Proteomics Data. .
520			_aThis volume provides an up-to-date compilation of current methodological approaches utilized for the exploration of nucleolar structure and function. Chapters cover a diversity of protocols that include imaging of the nucleolus, analysis of ribosomal RNA transcription and processing, and genomics and proteomics of the nucleolus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practical handbook to facilitate the investigation of this nuclear compartment at the advanced level.
700	1		_aNémeth, Attila _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493937905
776	0	8	_iPrinted edition: _z9781493937912
776	0	8	_iPrinted edition: _z9781493981403
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3792-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235164 _d235164