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008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597452298
024	7		_a10.1385/1597452297 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtocols for Nucleic Acid Analysis by Nonradioactive Probes _cedited by Elena Hilario, John F. MacKay.
250			_a2nd edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (321 páginas) _b66 ilustraciones, 5 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v353
505	0		_aNucleic Acid Extractions -- Genomic DNA Isolation From Different Biological Materials -- Extraction of Plant RNA -- Indirect Detection -- Overview of Hybridization and Detection Techniques -- Checkerboard DNA-DNA Hybridization Technology Using Digoxigenin Detection -- Nonradioactive Northern and Southern Analyses From Plant Samples -- Screening a BAC Library With Nonradioactive Overlapping Oligonucleotide (Overgo) Probes -- Direct In-Gel Hybridization of DNA With Digoxigenin-Labeled Probes -- In Situ Hybridization of Termite Microbes -- Fluorescent Labeling and Detection -- RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer -- Comparative Quantitation of mRNA Expression in the Central Nervous System Using Fluorescence In Situ Hybridization -- Visualization of Gene Expression by Fluorescent Multiplex Reverse Transcriptase-PCR Amplification -- Fluorescence In Situ Hybridization for the Identification of Environmental Microbes -- Kinetic ("Real-Time") PCR -- to Kinetic (Real-Time) PCR -- Validation of Short Interfering RNA Knockdowns by Quantitative Real-Time PCR -- Real-Time Quantitative PCR as an Alternative to Southern Blot or Fluorescence In Situ Hybridization for Detection of Gene Copy Number Changes -- Design and Work-Up of a New Molecular Diagnostic Assay Based on Real-Time PCR -- Real-Time PCR Fluorescent Chemistries -- Microarrays -- Microarrays -- Oligonucleotide Microarrays for the Study of Coastal Microbial Communities.
520			_aProtocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
700	1		_aHilario, Elena _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMacKay, John F _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617375644
776	0	8	_iPrinted edition: _z9781588294302
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597452297 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c235168 _d235168