000			03621nam a22003735i 4500
001			235169
003			ES-VaUE
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007			cr nn 008mamaa
008			120419s2012 xxu| s |||| 0|eng d
020			_a9781617796210
024	7		_a10.1007/978-1-61779-621-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aInfluenza Virus _bMethods and Protocols _cedited by Yoshihiro Kawaoka, Gabriele Neumann.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XI, 234 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v865
505	0		_aInfluenza Viruses - An Introduction -- Influenza Virus Isolation -- Influenza Virus Titration, Antigenic Characterization, and Serological Methods for Antibody Detection -- Diagnosis of Influenza Virus -- Solid-phase Assays of Receptor-binding Specificity -- The Chemiluminescent Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors -- The Fluorescence Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors -- Animal Models -- Influenza Virus Surveillance, Vaccine Strain Selection and Manufacture -- Genetic Engineering of Live Attenuated Influenza Viruses -- Influenza A Virus Molecular Virology Techniques -- Reverse Genetics of Influenza Viruses -- Genetic Analysis.
520			_aReports of influenza-like illnesses date back to the Middle Ages, and outbreaks of influenza likely afflicted humans long before that. Over the last half century, influenza virus research has led to the development of two classes of antivirals - ion channel and neuraminidase inhibitors. Recently, a method of the artificial generation of an influenza virus was established. This system has been instrumental in the development of novel influenza vaccines and in the understanding of viral pathogenicity and the functions of viral proteins. Influenza Virus: Methods and Protocols summarizes the current techniques that have made this progress possible, ranging from protocols for virus isolation, growth, and subtyping to procedures for the efficient generation of any influenza virus. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Influenza Virus: Methods and Protocols seeks to serve both professionals and novices with the techniques used in numerous laboratories around the world that are, thus, the building blocks that underpin almost all influenza virus research.
700	1		_aKawaoka, Yoshihiro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNeumann, Gabriele _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617796203
776	0	8	_iPrinted edition: _z9781617796227
776	0	8	_iPrinted edition: _z9781493957682
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-621-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c235169 _d235169