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001			235182
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007			cr nn 008mamaa
008			100301s2008 xxu| s |||| 0|eng d
020			_a9781597452465
024	7		_a10.1007/978-1-59745-246-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNew Antibiotic Targets _cedited by W. Scott Champney.
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XII, 274 páginas) _b51 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v142
505	0		_aBiocomputational Strategies for Microbial Drug Target Identification -- Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities -- A Method to Assay Inhibitors of DNA Polymerase IIIC Activity -- Methods to Identify and Characterize Inhibitors of Bacterial RNA Polymerase -- Methods to Assay Inhibitors of tRNA Synthetase Activity -- Three Methods to Assay Inhibitors of Ribosomal Subunit Assembly -- Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis -- Assays for the Identification of Inhibitors Targeting Specific Translational Steps -- SPARK: A New Peptidyl Transferase Activity Assay -- High-Throughput Screening of Peptide Deformylase Inhibitors -- A Method to Assay Penicillin-Binding Proteins -- A Method to Assay Inhibitors of Lipopolysaccharide Synthesis -- Methods for Assessing the Structure and Function of Cationic Antimicrobial Peptides -- Flow Cytometry of Bacterial Membrane Potential and Permeability -- Bacterial Efflux Pump Inhibitors -- Mycobacterium tuberculosis ?-Ketoacyl Acyl Carrier Protein Synthase III (mtFabH) Assay: Principles and Method -- Screening for Compounds That Affect the Interaction Between Bacterial Two-Component Signal Transduction Response Regulator Protein and Cognate Promoter DNA -- The Activity of rRNA Resistance Methyltransferases Assessed by MALDI Mass Spectrometry -- Assays for ?-Lactamase Activity and Inhibition -- Studies of Enzymes That Cause Resistance to Aminoglycosides Antibiotics.
520			_aA crisis is developing in the treatment of infectious diseases, with microorganisms frequently showing resistance to currently prescribed antibiotics. Fewer and fewer antimicrobial agents are now available to treat these infections. In "New Antibiotic Targets," W. Scott Champney examines specific techniques which can be used to explore new drug targets and the effectiveness of new antibiotics. By testing new antimicrobial agents and modified existing drugs, the most vulnerable cell processes, such as cell wall and membrane synthesis, DNA replication, RNA transcription and protein synthesis, can be better exploited. This in-depth volume, however, delves even deeper by identifying additional novel cellular targets for these new therapies. With detailed methods and protocols for the identification and assay of these targets, "New Antibiotic Targets" provides laboratory investigators with the vital tools they need to test the antimicrobial potential of products and to curb the rise of so many infectious diseases.
700	1		_aChampney, W. Scott _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617378430
776	0	8	_iPrinted edition: _z9781588299154
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-246-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2008
999			_c235182 _d235182