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007			cr nn 008mamaa
008			100715s2010 xxu| s |||| 0|eng d
020			_a9781597455459
024	7		_a10.1007/978-1-59745-545-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCancer Gene Profiling _bMethods and Protocols _cedited by Robert Grützmann, Christian Pilarsky.
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XII, 452 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v576
505	0		_aOrganizational Issues in Providing High-Quality Human Tissues and Clinical Information for the Support of Biomedical Research -- Manual Microdissection -- Laser Microdissection -- Tissue Microarrays -- A Decade of Cancer Gene Profiling: From Molecular Portraits to Molecular Function -- Mining Expressed Sequence Tag (EST) Libraries for Cancer-Associated Genes -- Automated Fluorescent Differential Display for Cancer Gene Profiling -- Manual Microdissection Combined with Antisense RNA-LongSAGE for the Analysis of Limited Cell Numbers -- Quantitative DNA Methylation Profiling on a High-Density Oligonucleotide Microarray -- Single-Nucleotide Polymorphism (SNP) Analysis to Associate Cancer Risk -- Application of Proteomics in Cancer Gene Profiling: Two-Dimensional Difference in Gel Electrophoresis (2D-DIGE) -- Search for and Identification of Novel Tumor-Associated Autoantigens -- Integrative Oncogenomic Analysis of Microarray Data in Hematologic Malignancies -- Cancer Gene Profiling in Pancreatic Cancer -- Cancer Gene Profiling in Prostate Cancer -- Cancer Gene Profiling for Response Prediction -- The EGFR Pathway as an Example for Genotype: Phenotype Correlation in Tumor Genes -- Quantitation of CD39 Gene Expression in Pancreatic Tissue by Real-Time Polymerase Chain Reaction -- Functional Profiling Methods in Cancer -- Calibration of Microarray Gene-Expression Data -- Meta-analysis of Cancer Gene-Profiling Data -- Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment.
520			_aOver the last few years, the methods for analyzing cancer-related genes on a molecular level have changed rapidly. The advent of incredible new technology, such as automated sequencing, has served as a catalyst for many advances in the area of gene profiling, and has enabled the research community to investigate populations of cells in a given tissue. Cancer Gene Profiling: Methods and Protocols examines the world of cancer gene profiling in light of these new advances, providing highly developed microarray techniques alongside modern methods for arraying tissues and proteomics. Chapters bring together the experiences of leading scientists in each method of profiling, providing a clear overview of the ground-breaking techniques used to perform these experiments effectively. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Cancer Gene Profiling: Methods and Protocols is a fundamental guidebook for researchers in the field of cancer gene profiling, as well as for those individuals in the area of biomedical/translational research.
700	1		_aGrützmann, Robert _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPilarsky, Christian _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607612179
776	0	8	_iPrinted edition: _z9781934115763
776	0	8	_iPrinted edition: _z9781617796593
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-545-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2010
999			_c235185 _d235185