000			03526nam a22003735i 4500
001			235189
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s1999 xxu| s |||| 0|eng d
020			_a9781592596874
024	7		_a10.1385/1592596878 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCytotoxic Drug Resistance Mechanisms _cedited by Robert Brown, Uta Böger-Brown.
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XI, 238 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v28
505	0		_aDrug Resistance -- Cell Sensitivity Assays -- Cell Sensitivity Assays -- Cell Sensitivity Assays -- Analysis of Apoptosis in Tissue Sections -- Measurement of P-glycoprotein Function -- Measuring MDR-1 by Quantitative RT-PCR -- Microtiter Plate Technique for the Measurement of Glutathione in Fresh and Cryopreserved Lymphoblasts Using the Enzyme Recycling Method -- Measurement of Reduced Glutathione Using High-Pressure Liquid Chromatography -- Topoisomerase I and II Activity Assays -- 5-Fluorouracil Metabolizing Enzymes -- Measuring DNA Adducts by Immunoassay (ELISA) -- Measuring Drug-DNA Adducts in Individual Cells -- Measurement of Drug-Induced DNA Interstrand Crosslinking Using the Single-Cell Gel Electrophoresis (Comet) Assay -- PCR Analysis of Microsatellite Instability -- O 6-Alkylguanine-DNA Alkyltransferase Assay -- Analysis of the p53 Status of Tumors -- Bcl-2 Family Immunohistochemistry -- Genetic Analysis of Drug Resistance by Fluorescence In Situ Hybridization -- Genetic Analysis of Drug Resistance by Reverse In Situ Hybridization.
520			_aIn Cytotoxic Drug Resistance Mechanisms, leading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. The protocols contain detailed instructions and extensive troubleshooting tips that allow researchers effectively to study a wide variety of drug resistance mechanisms, including aspects of drug-induced cell death, drug uptake/efflux, drug metabolism, and DNA repair. Each method is designed to help identify the correlation between molecular and biochemical data and the clinical responses of the patient. Cytotoxic Drug Resistance Mechanisms makes it possible to test established and laboratory-derived hypotheses with clinically derived tumor samples. State-of-the-art and readily reproducible, the methods presented here afford basic and clinical scientists a powerful complement of tools for investigating all the clinically relevant mechanisms used as markers of the biological response to anticancer chemotherapeutics today.
700	1		_aBrown, Robert _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBöger-Brown, Uta _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617370939
776	0	8	_iPrinted edition: _z9780896036031
776	0	8	_iPrinted edition: _z9781489941725
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596878 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1999
999			_c235189 _d235189