000			04040nam a22003615i 4500
001			235190
003			ES-VaUE
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007			cr nn 008mamaa
008			151013s2016 xxu| s |||| 0|eng d
020			_a9781493930678
024	7		_a10.1007/978-1-4939-3067-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPost-Transcriptional Gene Regulation _cedited by Erik Dassi.
250			_a2nd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XIII, 353 páginas) _b43 ilustraciones, 30 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1358
505	0		_aIntroduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression -- A Computational Approach for the Discovery of Protein-RNA Networks -- Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells -- Studying the Translatome with Polysome Profiling -- Exploring Ribosome Positioning on Translating Transcripts with Ribosome Profiling -- Studying Isoform-Specific Recruitment to Polyribosomes with Frac-Seq -- Use of the Pbutr Reporter System for Scalable Analysis of 3' UTR-Mediated Gene Regulation -- Comprehensive Identification of RNA-Binding Proteins by RNA Interactome Capture -- Identifying RBP Targets with RIP-Seq -- PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites -- Profiling the Binding Sites of RNA-Binding Proteins with Nucleotide Resolution Using Iclip -- A Pipeline for PAR-CLIP Data Analysis -- Capture and Identification Of Mirna Targets By Biotin Pulldown And RNA-Seq -- Identification of Mirna-Target RNA Interactions Using CLASH -- Genome-Wide Analysis of A-To-I RNA Editing -- Nucleotide-Level Profiling Of M5c RNA Methylation -- Probing N6-Methyladenosine (M6a) RNA Modification in Total RNA with SCARLET -- Genome-Wide Identification of Alternative Polyadenylation Events Using 3'T-Fill -- Genome-Wide Profiling Of Alternative Translation Initiation Sites -- Genome-Wide Study Of Mrna Isoform Half-Lives -- Visualizing Mrna Dynamics in Live Neurons and Brain Tissues -- Single Molecule Live-Cell Visualization of Pre-Mrna Splicing.
520			_aThis volume aims to provide the most recent advances in techniques for studying gene expression regulation at the post-transcriptional level. Post-Transcriptional Gene Regulation, Second Edition is organized in six sections describing bioinformatics approaches for studying post-transcriptional regulation, various expression profiling approaches, protein-RNA interaction and non-coding RNA investigation techniques, methods for profiling RNA modifications, and other techniques such as alternative translation initiation or polyadenylation sites determination. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Post-Transcriptional Gene Regulation, Second Edition  is a versatile resource to researchers studying post-transcriptional regulation, introducing the most recent techniques and providing a comprehensive guide to their implementation.
700	1		_aDassi, Erik _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493930661
776	0	8	_iPrinted edition: _z9781493930685
776	0	8	_iPrinted edition: _z9781493949694
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3067-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235190 _d235190