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001			235193
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007			cr nn 008mamaa
008			100301s1994 xxu| s |||| 0|eng d
020			_a9781592595143
024	7		_a10.1385/0896032507 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
100	1		_aGraham, John M. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aBiomembrane Protocols _bII. Architecture and Function _cby John M. Graham, Joan A. Higgins.
250			_a1st edition 1994
264		1	_aTotowa, NJ _bHumana Press _c1994
300			_a1 recurso en línea (XIII, 362 páginas) _b7 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v27
505	0		_aCrystallization of Membrane Proteins for X-Ray Analysis -- Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination -- Biochemical Methods to Determine Cell-Surface Topography -- Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins -- The Production of Monoclonal Antibodies to Membrane Proteins -- Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles -- Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy -- Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy -- Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes -- Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools -- Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry -- Fluorescent Glycerolipid Probes -- Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids -- Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes -- Extraction and Assay of Cyclic Nucleotides -- Analysis of G-Proteins Regulating Signal Transduction Pathways -- Assay of Protein Kinases and Protein Phosphorylation -- Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Procedures -- Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatography -- Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes -- Membrane Permeabilization with Bacterial Toxins -- Measurement of Ion Fluxes and pH Gradients Across Cell Membranes -- Ligand Binding and Processing -- The Binding of Protein-Ligands to Cell-Surface Receptors.
520			_aThere have been many important advances in our understanding of biological membrane structure and function over the last decade. Much of this progress has been driven by the development of new techniques for studying membrane components and their interactions. Tradition­ ally, the investigation of membranes has largely occurred within the domains of biochemistry, physical chemistry, and cell biology; but many of the most significant advances have resulted from an expansion into other disciplines, such as molecular biology, immunology, and the clinical sciences. In these two volumes we have attempted to bring together some of these techniques-to combine the old and the new. Biomembrane Protocols: II. Architecture and Function is concerned exclusively with the architecture and activities of membranes, whereas the companion volume, Biomembrane Protocols: I. Isolation and Analysis, deals with their isolation and compositional analysis. The aim of each chapter is to provide detailed technical and meth­ odological information that will allow the reader to perform the tech­ nique successfully, without the need to consult other texts. Detailed reviews of membrane structure and function are not included, except where they are relevant to the choice or efficacy of a particular proce­ dure. In addition to Materials and Methods sections, each chapter has a Notes section that explains the reasons for taking certain steps and pro­ vides practical tips for applying the techniques to other systems.
700	1		_aHiggins, Joan A. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
776	0	8	_iPrinted edition: _z9781489939098
776	0	8	_iPrinted edition: _z9781489939081
776	0	8	_iPrinted edition: _z9780896032507
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032507 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1994
999			_c235193 _d235193