000			03625nam a22003615i 4500
001			235194
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592591749
024	7		_a10.1385/1592591744 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aThyroid Hormone Receptors _bMethods and Protocols _cedited by Aria Baniahmad.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (X, 223 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v202
505	0		_ato Thyroid Hormone Receptors -- Null Mutant Mice for Thyroid Hormone Receptors -- Transgenic Targeting of a Dominant Negative Corepressor to Liver and Analyses by cDNA Microarray -- The Role of Thyroid Hormone Receptors in the Heart -- Analysis of Thyroid Hormone-Dependent Genes in the Brain by In Situ Hybridization -- The v-erbA Oncogene -- Gene Regulation by Thyroid Hormone Receptors -- Molecular Analysis of Human Resistance to Thyroid Hormone Syndrome -- Function of Thyroid Hormone Receptors During Amphibian Development -- Transcriptional Regulation by Thyroid Hormone Receptor in Chromatin -- Gene Activation by Thyroid Hormone Receptor In Vitro and Purification of the TRAP Coactivator Complex.
520			_aBecause thyroid hormone receptors (TRs) are important regulators of a large number of biological processes-ranging from brain development to control of vision in vertebrates-abnormal functioning of these receptors can have serious medical consequences. In Thyroid Hormone Receptors: Methods and Protocols, a panel of outstanding investigators surveys and explains the major cutting-edge methods used in TR research and their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TRs in development and generation of knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for the study of blood cell differentiation through the oncogene v-erbA, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver. Each protocol has been developed by a hands-on expert and contains full explanatory notes, troubleshooting tips, and helpful comments on avoiding pitfalls. Broad ranging and highly practical, Thyroid Hormone Receptors: Methods and Protocols offers endocrinologists, geneticists, biochemists, and research physicians a comprehensive collection of powerful methods useful not only for all major areas of TR biology and analysis, but also for nuclear hormone receptors in general.
700	1		_aBaniahmad, Aria _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489938794
776	0	8	_iPrinted edition: _z9781489938787
776	0	8	_iPrinted edition: _z9780896039957
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591744 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c235194 _d235194