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008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597454902
024	7		_a10.1007/978-1-59745-490-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMolecular Motors _bMethods and Protocols _cedited by Ann O. Sperry.
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XII, 252 páginas) _b37 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v392
505	0		_aEquilibrium Binding of Proteins to F-Actin -- Analysis of Calcium/Calmodulin Regulation of a Plant Kinesin Using Co-Sedimentation and ATPase Assays -- In Vitro and In Vivo Analysis of Microtubule-Destabilizing Kinesins -- Approaches to Kinesin-1 Phosphorylation -- Protein Modification to Probe Intradynein Interactions and In Vivo Redox State -- Methods to Study the Interactions of the Dynein Light Chains and Intermediate Chains -- Identification of Motor Protein Cargo by Yeast 2-Hybrid and Affinity Approaches -- In Situ Binding Assay to Detect Myosin-1c Interactions with Hair-Cell Proteins -- Ultrastructural Analysis of Kinesin-Related Motor Proteins During Spermatogenesis -- In Vitro Motility System to Study the Role of Motor Proteins in Receptor-Ligand Sorting -- Enrichment and Disassembly of Ectoplasmic Specializations in the Rat Testis -- Single-Molecule Observation of Rotation of F1-ATPase Through Microbeads -- The Use of FRET in the Analysis of Motor Protein Structure -- Structure Determination of the Motor Domain of Yeast Kinesin Kar3 by X-Ray Crystallography -- High-Resolution Structural Analysis of the Kinesin-Microtubule Complex by Electron Cryo-Microscopy -- Chemical-Genetic Inhibition of Sensitized Mutant Unconventional Myosins.
520			_aMolecular motor proteins comprise three protein superfamilies: kinesins, dyneins and myosins. Together, these proteins produce force for movement in an incredibly wide variety of cellular processes including vesicular transport, cell division, nuclear migration, muscle contraction, mechanochemical transduction, among others. In addition, molecular motors are expressed in both simple eukaryotes and in a tissue specific manner in higher cells. This methods volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the 3 characterized families (i.e. dynamin and F1ATPase). This volume of the Methods in Molecular Biology series is an essential reference for cell biologists examining the function of motor proteins in a range of model organisms using both in vitro and in vivo techniques. This volume is a valuble source for biochemists and molecular biologists as well.
700	1		_aSperry, Ann O _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617377068
776	0	8	_iPrinted edition: _z9781588296658
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-490-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c235195 _d235195