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001			235199
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007			cr nn 008mamaa
008			160810s2016 xxu| s |||| 0|eng d
020			_a9781493936229
024	7		_a10.1007/978-1-4939-3622-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Cytogenetics _bMethods and Protocols _cedited by Shahryar F. Kianian, Penny M. Avoles Kianian.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XII, 198 páginas) _b40 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1429
505	0		_aC-Banding of Plant Chromosomes -- Chromosome Painting by GISH and Multi-Color FISH -- Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant Chromosomes -- Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homologous Chromosomes -- Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH -- Immunolabeling and In Situ Labelling of Isolated Plant Interphase Nuclei -- Manipulation of Homologous Chromosome Recombination in Wheat -- Dissecting Plant Chromosomes by the Use of Ionizing Radiation -- Optical Nano-Mapping and Analysis of Plant Genomes -- Flow Sorting Plant Chromosomes -- Construction of BAC Libraries from Flow-Sorted Chromosomes -- The Chromosome Microdissection and Microcloning Technique -- Immunolocalization on Whole Anther Chromosome Spreads for Male Meiosis -- Mapping Recombination Initiation Sites Using Chromatin Immunoprecipitation -- Chromatin Immunoprecipitation to Study Plant Epigenome.
520			_aThis volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics. .
700	1		_aKianian, Shahryar F _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKianian, Penny M. Avoles _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493936205
776	0	8	_iPrinted edition: _z9781493936212
776	0	8	_iPrinted edition: _z9781493980970
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3622-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c235199 _d235199