000			04055nam a22003615i 4500
001			235200
003			ES-VaUE
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007			cr nn 008mamaa
008			101120s2011 xxu| s |||| 0|eng d
020			_a9781617379703
024	7		_a10.1007/978-1-61737-970-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCell-Based Microarrays _bMethods and Protocols _cedited by Ella Palmer.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (X, 209 páginas) _b46 ilustraciones, 10 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v706
505	0		_aCell-Based Microarrays: Overview -- Cell-Based Co-Transfection Microarrays for Use with HEK293T Cells on a Poly D-Lysine Polystyrene Coated Microplate -- Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays -- A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays -- High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays -- Cell Arrays for the Measurement of Organelle Dynamics in Living Cells -- High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays -- Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA) -- Reverse Transfected Cell Microarrays in Infectious Disease Research -- Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies -- Blood Cell Capture on Antibody Micro-Arrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging -- Immobilized Culture and Transfection Microarray of Nonadherent Cells -- Plasma Polymer and PEG-Based Coatings for DNA, Protein, and Cell Microarrays -- Polymer Microarrays for Cellular High-Content Screening -- High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation -- Microfluidic Image Cytometry.
520			_aAs a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.
700	1		_aPalmer, Ella _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617379697
776	0	8	_iPrinted edition: _z9781617379710
776	0	8	_iPrinted edition: _z9781493957842
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61737-970-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c235200 _d235200