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020 _a9781617792311
024 7 _a10.1007/978-1-61779-231-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aSeed Dormancy
_bMethods and Protocols
_cedited by Allison R. Kermode.
250 _a1st edition 2011
264 1 _aTotowa, NJ
_bHumana Press
_c2011
300 _a1 recurso en línea (XVI, 415 páginas)
_b85 ilustraciones, 1 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v773
505 0 _aChallenges Facing the Forest Industry in Relation to Seed Dormancy and Seed Quality -- Challenges Facing Seed Banks and Agriculture in Relation to Seed Quality -- Standardizing Seed Dormancy Research -- Eyeing Emergence: Modified Treatments for Terminating Dormancy of Conifer Seeds -- Production of Seed Samples for the Effective Molecular Analysis of Dormancy Cycling in Arabidopsis -- Approaches to the Identification of ABAR as an Abscisic Acid Receptor -- Profiling of Hormones and Related Metabolites in Seed Dormancy and Germination Studies -- In Vitro Assay for ABA 8'-Hydroxylase: Implications for Improved Assays for Cytochrome P450 Enzymes -- Functional Analysis of Abscisic Acid 8'-Hydroxylase -- Isolation of Genetic Material from Arabidopsis Seeds -- Identification and Characterization of Quantitative Trait Loci (QTL) that Control Dormancy in Arabidopsis -- Identification of Seed Dormancy Mutants by Activation Tagging -- Isolation of MicroRNAs that Regulate Seed Dormancy and Germination -- Proteomics and Post-Translational Proteomics of Seed Dormancy and Germination -- Chromatin Dynamics during Seed Dormancy -- Emerging Mass Spectrometry-Based Technologies for Analyses of Chromatin Changes:  Analysis of Histones and Histone Modifications -- In Vivo Nuclear Magnetic Resonance (NMR) Metabolite Profiling in Plant Seeds -- In Vivo 1H NMR Microimaging during Seed Imbibition, Germination, and Early Growth -- Tissue Printing Methods for Localization of RNA and Proteins that Control Seed Dormancy and Germination -- Analyses of Reactive Oxygen Species and Antioxidants in Relation to Seed Longevity and Germination -- Protein Damage and Repair Controlling Seed Vigor and Longevity -- Nitric Oxide Methods in Seed Biology -- Seed Bioinformatics.
520 _aUnderstanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world's food supply. Seed Dormancy: Methods and Protocols covers analytical methods and approaches which have already lead to significant advances in the understanding of seed dormancy and germination. Chapters cover explanations of processes leading to the induction, maintenance, and termination of seed dormancy, the classification of different dormancy types, as well as an overview of protocols used for dormancy-termination of seeds of conifer species. This volume emphasizes methods essential for abscisic acid (ABA) analyses, including methods that have been important for receptor identification, analyses of ABA-catabolizing enzymes (the 8'-hydroxylases), and identification of novel signal transduction components, interacting partners, and/or response factors. The volume closes by addressing the development of new technologies, including spectroscopic methods (some of which allow for non-destructive sampling) as well as highly effective tissue-printing methods for seed dormancy research. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Seed Dormancy: Methods and Protocols features detailed methods that will prove invaluable for both applied and fundamental seed research.
700 1 _aKermode, Allison R
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617792304
776 0 8 _iPrinted edition:
_z9781617792328
776 0 8 _iPrinted edition:
_z9781493961399
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-231-1
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2011
999 _c235201
_d235201