000			04315nam a22003735i 4500
001			235205
003			ES-VaUE
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007			cr nn 008mamaa
008			150316s2015 xxu| s |||| 0|eng d
020			_a9781493924509
024	7		_a10.1007/978-1-4939-2450-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMycobacteria Protocols _cedited by Tanya Parish, David M. Roberts.
250			_a3rd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XII, 416 páginas) _b88 ilustraciones, 37 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1285
505	0		_aWhole Genome Sequencing for Comparative Genomics and De Novo Genome Assembly -- Whole Transcriptome Sequencing for High-resolution Transcriptomic Analysis in Mycobacterium tuberculosis -- RNA Sequencing for Transcript 5'-end Mapping in Mycobacteria -- Fractionation and Analysis of Mycobacterial Proteins -- Lipid and Lipoarabinomannan Isolation and Characterization -- Metabolomics of Mycobacterium tuberculosis -- Electroporation of Mycobacteria -- Targeted Gene Knockout and Essentiality Testing by Homologous Recombination -- Construction of Conditional Knockdown Mutants in Mycobacteria -- Mycobacterial Recombineering -- In vitro Models that Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria -- Genetic Dissection of Mycobacterial Biofilms -- Measuring Efflux and Permeability in Mycobacteria -- Single-cell Analysis of Mycobacteria using Microfluidics and Time-lapse Microscopy -- Antimicrobial Susceptibility Testing for Mycobacterium sp. -- Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis -- Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis -- A Multi-Stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis -- Isolation and Characterization of Compound-resistant Isolates of Mycobacterium tuberculosis -- Macrophage Infection Models for Mycobacterium tuberculosis -- Infection of Human Neutrophils to Study Virulence Properties of Mycobacterium tuberculosis -- Isolation of Bead-phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids -- Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum -- Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells using Phenotypic Assays.  .
520			_aUpdated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory.  Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites.  Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field, and to newcomers.
700	1		_aParish, Tanya _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRoberts, David M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493924516
776	0	8	_iPrinted edition: _z9781493924493
776	0	8	_iPrinted edition: _z9781493955824
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2450-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c235205 _d235205