000			03560nam a22003735i 4500
001			235206
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s1998 xxu| s |||| 0|eng d
020			_a9781592595785
024	7		_a10.1385/0896034216 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPichia Protocols _cedited by David R. Higgins, James Cregg.
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XI, 270 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v103
505	0		_ato Pichia pastoris -- Classical Genetic Manipulation -- Transformation -- Small Vectors for Expression Based on Dominant Drug Resistance with Direct Multicopy Selection -- The Generation of Multicopy Recombinant Strains -- Isolation of Nucleic Acids -- Generation of Protease-Deficient Strains and Their Use in Heterologous Protein Expression -- Glycosylation Profiling of Heterologous Proteins -- High Cell-Density Fermentation -- Use of Pichia pastoris as a Model Eukaryotic System -- Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I) -- Secretion of scFv Antibody Fragments -- Expression of Tetanus Toxin Fragment C -- Expression of EGF and HIV Envelope Glycoprotein -- Expression of an Integral Membrane Protein, the 5HT5A Receptor -- Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections.
520			_aThe Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors, and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Pichia Protocols captures the collective expertise of the international P. pastoris scientific community in close detail. It conveys a breadth of experience that will permit any researcher in molecular biology to quickly construct an expression strain designed to produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.
700	1		_aHiggins, David R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aCregg, James _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489928023
776	0	8	_iPrinted edition: _z9781489928016
776	0	8	_iPrinted edition: _z9780896034211
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034216 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1998
999			_c235206 _d235206