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007			cr nn 008mamaa
008			141118s2015 xxu| s |||| 0|eng d
020			_a9781493919024
024	7		_a10.1007/978-1-4939-1902-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Cell Expansion _bMethods and Protocols _cedited by José M. Estevez.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 231 páginas) _b49 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1242
505	0		_aPenium margaritaceum as a Model Organism For Cell Wall Analysis Of Expanding Plant Cells -- Using Chemical Genomics to Study Cell Wall Formation and Cell Growth in Arabidopsis thaliana and Penium margaritaceum -- Optimized Method For Growing In Vitro arabidopsis Thaliana Pollen Tubes -- Imaging of Calcium Dynamics in Pollen Tube Cytoplasm -- Live Imaging of Root Hairs -- Improved ROS Measurement In Root Hair Cells -- A Root Hair Assay to Expedite Cell Death Research -- Vacuolar Staining Methods in Plant Cells -- Live Cell Imaging Of FM4-64, A Tool For Tracing The Endocytic Pathways In Arabidopsis Root Cells -- Salt-Stress Regulation Of Root System Growth And Architecture In Arabidopsis Seedlings -- Quantification Of Fluorescent Reporters in Plant Cells -- Live Cell Imaging Of The Cytoskeleton And Cell Wall Enzymes In Plant Cells -- Using the Split-Ubiquitin Yeast Two-Hybrid System to Test Protein-Protein Interactions of Transmembrane Proteins -- Activation Tag Screening For Cell Expansion Genes in Arabidopsis thaliana -- Bifc For Protein-Protein Interactions and Protein Topology: Discussing An Integrative Approach For An Old Technique -- N-Glycosylation and Plant Cell Growth -- Peptide Separation Methodologies for In-depth Proteomics -- Structural And Mechanical Characterization Of Growing Arabidopsis< Plant Cell Walls.
520			_aThis volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists .
700	1		_aEstevez, José M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493919031
776	0	8	_iPrinted edition: _z9781493919017
776	0	8	_iPrinted edition: _z9781493953813
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1902-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c235210 _d235210