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001			235216
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007			cr nn 008mamaa
008			100301s2008 xxu| s |||| 0|eng d
020			_a9781597452427
024	7		_a10.1007/978-1-59745-242-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAlcohol _bMethods and Protocols _cedited by Laura E. Nagy.
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XX, 420 páginas) _b74 ilustraciones, 2 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v447
505	0		_aAnimal Models of Acute and Chronic Ethanol Exposure -- Acute Models of Ethanol Exposure to Mice -- A Voluntary Oral-feeding Rat Model for Pathological Alcoholic Liver Injury -- Intragastric Ethanol Infusion Model in Rodents -- A Practical Method of Chronic Ethanol Administration in Mice -- Animal Models of Ethanol Exposure During Development -- Analysis of Ethanol Developmental Toxicity in Zebrafish -- The Avian Embryo in Fetal Alcohol Research -- Artificial Rearing -- Intragastric Intubation of Alcohol During the Perinatal Period -- Cell Culture Approaches to Studying Ethanol Exposure -- Human Monocytes, Macrophages, and Dendritic Cells: Alcohol Treatment Methods -- Generation and Use of Primary Rat Cultures for Studies of the Effects of Ethanol -- Development and Properties of HepG2 Cells That Constitutively Express CYP2E1 -- Modeling the Impact of Alcohol on Cortical Development in a Dish: Strategies from Mapping Neural Stem Cell Fate -- Regulation of Specific Organ Systems in Response to Ethanol -- Acetaldehyde-induced Barrier Disruption and Paracellular Permeability in Caco-2 Cell Monolayer -- Alcohol-Induced Oxidative Stress in the Liver: In Vivo Measurements -- Isolation of Kupffer Cells from Rats Fed Chronic Ethanol -- Dendritic Cells in Chronic In Vivo Ethanol Exposure Models -- Formation and Immunological Properties of Aldehyde-derived Protein Adducts following Alcohol Consumption -- Assessment of Natural Killer (NK) and NKT Cells in Murine Spleens and Livers -- Polyclonal and Antigen-specific Responses of T Cells and T Cell Subsets -- B-Cell Studies in Chronic Ethanol Mice -- Histological Analysis of Bone -- Assessing Effects of Alcohol Consumption on Protein Synthesis in Striated Muscles -- Methods to Investigate the Effects of Chronic Ethanol on Adipocytes -- Proteomic and Genomic Approaches to Study Response to Ethanol -- Proteomic Approaches to Identify and Characterize Alterations to the Mitochondrial Proteome in Alcoholic Liver Disease -- Alcoholic Liver Disease and the Mitochondrial Ribosome: Methods of Analysis -- Microarray Analysis of Ethanol-Induced Changes in Gene Expression.
520			_aMany unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics. As part of the successful Methods in Molecular Biology™ series, each chapter provides step-by-step laboratory protocols, lists of the necessary materials, and a Notes section with tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly specific, Alcohol: Methods and Protocols strives to further spur investigators to delve into the complex and fascinating processes of the adaptive and maladaptive responses humans have developed to the consumption of alcohol.
700	1		_aNagy, Laura E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617378379
776	0	8	_iPrinted edition: _z9781588299062
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-242-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2008
999			_c235216 _d235216