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001			235217
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007			cr nn 008mamaa
008			110516s2011 xxu| s |||| 0|eng d
020			_a9781617790959
024	7		_a10.1007/978-1-61779-095-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aViral Vectors for Gene Therapy _bMethods and Protocols _cedited by Otto-Wilhelm Merten, Mohamed Al-Rubeai.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 450 páginas) _b62 ilustraciones, 5 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v737
505	0		_aIntroduction to Viral Vectors -- Introduction to Gene Therapy: A Clinical Aftermath -- Host Cells and Cell Banking -- Overview of Current Scalable Methods for Purification of Viral Vectors -- Methods to Construct Recombinant Adenovirus Vectors -- Manufacturing of Adenoviruses Vectors: Production and Purification of Helper Dependent Adenovirus -- Manufacturing of Retroviruses -- Lentiviral Vectors -- Adeno-Associated Viruses -- Manufacturing of Adeno-Associated Viruses, for Example: AAV2 -- Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses -- Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant and Amplicon Vectors -- Manufacture of Measles Viruses -- In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model -- In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (in View of Cartilage Repair) -- Ethical Consideration -- Clinical Trials of GMP Products in the Gene Therapy Field.
520			_aThe huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.  In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures.  Written in the highly successful Methods in Molecular Biology™ series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.   Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.
700	1		_aMerten, Otto-Wilhelm _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aAl-Rubeai, Mohamed _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617790942
776	0	8	_iPrinted edition: _z9781617790966
776	0	8	_iPrinted edition: _z9781493958283
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-095-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c235217 _d235217