000			03556nam a22003615i 4500
001			235218
003			ES-VaUE
005			20221220020651.0
007			cr nn 008mamaa
008			100301s1999 xxu| s |||| 0|eng d
020			_a9781592596782
024	7		_a10.1385/1592596789 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMolecular Methods in Developmental Biology _bXenopus and Zebrafish _cedited by Matt Guille.
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XII, 217 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v127
505	0		_aThe Animal Cap Assay -- Cell and Tissue Transplantation in Zebrafish Embryos -- Ribonuclease Protection Analysis of Gene Expression in Xenopus -- Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) -- Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos -- In Situ Hybridization to Sections of Xenopus Embryos -- Zebrafish Immunohistochemistry -- Immunohistochemistry of Xenopus Embryos -- Preparation and Testing of Synthetic mRNA for Microinjection -- Microinjection into Xenopus Oocytes and Embryos -- Microinjection into Zebrafish Embryos -- Expression from DNA Injected into Xenopus Embryos -- Promoter Analysis in Zebrafish Embryos -- Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs -- Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis -- DNA-Footprinting using Crude Embryonic Extracts from Xenopus laevis -- Mapping Protein-DNA Interactions Using In Vivo Footprinting.
520			_aIn Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Wherever possible, these experimental approaches are applied to both Xenopus and zebrafish. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have fine-tuned them for best results, and a plethora of time-saving tips. State-of-the-art and readily reproducible, these powerful methods constitute today's gold-standard laboratory manual for understanding the interactions responsible for development.
700	1		_aGuille, Matt _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371899
776	0	8	_iPrinted edition: _z9781489943316
776	0	8	_iPrinted edition: _z9780896037908
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596789 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1999
999			_c235218 _d235218