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008 110108s2011 xxu| s |||| 0|eng d
020 _a9781617790089
024 7 _a10.1007/978-1-61779-008-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 4 _aThe Plant Cell Wall
_bMethods and Protocols
_cedited by Zoë Popper.
250 _a1st edition 2011
264 1 _aTotowa, NJ
_bHumana Press
_c2011
300 _a1 recurso en línea (XIII, 310 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v715
505 0 _aPlant Tissue Cultures -- Computerized Molecular Modelling of Carbohydrates -- Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS -- High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors -- Carbohydrate Gel Electrophoresis -- Capillary Electrophoresis with Detection by Laser-Induced Fluorescence -- Monoclonal antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls -- Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays -- Electron Tomography and Immunogold Labelling as Tools to Analyze de novo Assembly of Plant Cell Walls -- Analyzing Cellulose Biosynthesis with Confocal Microscopy -- Visual Mapping of Cell Wall Biosynthesis -- Atomic Force Microscopy of Plant Cell Walls -- Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls -- Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Gluconacetobacter Fermentation -- Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis -- New Insights into the Control of Cell Growth -- Extraction and Detection of Arabinogalactan Proteins -- Characterization of the Plant Cell Wall Proteome Using High Throughput Screens -- Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens -- Measuring In vitro Extensibility of Growing Plant Cell Walls.
520 _aThe cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions. In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism.  The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedures which measure the physical properties of the wall, and methods based on the application of molecular genetic approaches.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, The Plant Cell Wall: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.
700 1 _aPopper, Zoë
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617790072
776 0 8 _iPrinted edition:
_z9781617790096
776 0 8 _iPrinted edition:
_z9781493961795
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-008-9
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2011
999 _c235219
_d235219