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001			235226
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007			cr nn 008mamaa
008			140911s2014 xxu| s |||| 0|eng d
020			_a9781493913633
024	7		_a10.1007/978-1-4939-1363-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aYeast Genetics _bMethods and Protocols _cedited by Jeffrey S. Smith, Daniel J. Burke.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 378 páginas) _b57 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1205
505	0		_aYeast Transformation by LiAc/SS Carrier DNA/PEG Method -- Tetrad, Random Spore and Molecular Analysis of Meiotic Segregation and Recombination -- PCR Mutagenesis and Gap Repair in Yeast -- PCR-Mediated Epitope Tagging of Genes in Yeast -- Manipulating the Yeast Genome: Deletion, Mutation and Tagging by PCR -- Preparation of Yeast Cells for Live Cell Imaging and Indirect Immunofluorescence -- Single Yeast Cell Imaging -- Microfluidic Platforms for Generating Dynamic Environmental Perturbations to Study the Responses of Single Yeast Cells -- Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations -- Synthetic Genetic Array Analysis of Global Mapping of Genetic Networks in Yeast -- Chemical Genetic and Chemogenomic Analysis in Yeast -- Phenomic Assessment of Genetic Buffering by Kinetic Analysis of Cell Arrays -- Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast -- Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast -- Global Analysis of Transcription Factor Binding Sites in Yeast Using ChIP-Seq -- High-Density Tiling Microarrays Analysis of the Full Transcriptional Activity of Yeast -- Analysis of Silencing in Saccharomyces cerevisiae -- A User's Guide to the Ribosomal DANN in Saccharomysces cerevisiae -- Two-Dimensional Agarose Gel Electrophoresis for Analysis of DNA Replication -- Replicative Life Span Alaysis in Budding Yeast -- Metabolomic and Lipidomic Analyses of Chronologically Aging Yeast.
520			_aYeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome, and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered, and tagged at will. Saccharomyces cerevisiae, has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription, and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.
700	1		_aSmith, Jeffrey S _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBurke, Daniel J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493913640
776	0	8	_iPrinted edition: _z9781493913626
776	0	8	_iPrinted edition: _z9781493951826
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1363-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c235226 _d235226